CONSTRUCTION OF A SACCHAROMYCES-CEREVISIAE STRAIN ABLE TO FERMENT CELLOBIOSE

被引：15

作者：

ADAM, AC ^{[1
]}

POLAINA, J ^{[1
]}

机构：

[1] CSIC,INST AGROQUIM & TECNOL ALIMENTOS,JAIME ROIG 11,E-46010 VALENCIA,SPAIN

来源：

CURRENT GENETICS | 1991年 / 20卷 / 1-2期

关键词：

BACILLUS-POLYMYXA; CELLOBIOSE; BETA-GLUCOSIDASE; HETEROLOGOUS GENE EXPRESSION;

D O I：

10.1007/BF00312758

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The bglA gene, encoding a beta-glucosidase from Bacillus polymyxa, has been expressed in Saccharomyces cerevisiae under control of the CYC-GAL promoter inducible by galactose. The expression of bglA-encoded activity in the strain used as a host was not sufficient to allow its growth with cellobiose as a carbon source. However, a recessive mutation in a gene designated cem1 has been obtained which, combined with the expression of beta-glucosidase activity, allows the growth of S. cerevisiae on cellobiose. The expression of the bglA gene in a cem1 strain confers on S. cerevisiae the capability for an efficient fermentation of cellobiose, as detected by the formation of CO2.

引用

页码：5 / 8

页数：4

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