AUTOMATED AMINO-ACID-ANALYSIS USING PRECOLUMN DERIVATIZATION WITH DANSYLCHLORIDE AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：43

作者：

SIMMACO, M

DEBIASE, D

BARRA, D

BOSSA, F

机构：

[1] UNIV LA SAPIENZA,DIPARTIMENTO SCI BIOCHIM,PIAZZALE A MORO 5,I-00185 ROME,ITALY

[2] UNIV LA SAPIENZA,CNR,CTR BIOL MOLEC,I-00185 ROME,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 504卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)89519-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An automated procedure for the precolumn derivatization of amino acids with dansylchloride and a liquid chromatographic method for separation of the derivatives with fluorimetric detection in the picomole range are reported. The method involves a simple solvent preparation, which does not require the inclusion of non-volatile buffers of low ionic strength and delicate pH adjustments. The procedure was also utilized for the identification of COOH-amidated amino acids released from peptides after digestion with carboxypeptidase. © 1990.

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页码：129 / 138

页数：10

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