SOIL BIOTIC AND BIOCHEMICAL FACTORS IN A LONG-TERM TILLAGE AND STUBBLE MANAGEMENT EXPERIMENT ON A VERTISOL .2. NITROGEN DEFICIENCY WITH ZERO TILLAGE AND STUBBLE RETENTION

The aim of this investigation was to find the cause of poor growth of barley that occurred with zero tillage and stubble retention in a long-term fallow management experiment on a vertisol in southern Queensland. The experiment compares 12 treatments comprising three factors of tillage (zero, mechanical), stubble (burnt, retained) and nitrogen fertilizer (0, 23 and 46 kg ha-1 year-1) in four randomised blocks. Dry matter yield of barley at anthesis showed a highly significant Mitscherlich relationship with tissue nitrogen (N) concentration. Cate-Nelson analysis indicated a critical N concentration of 1.58% N. The barley responded to fertilizer N although 9 of 12 zero-till, stubble-retained plots and 5 of 12 mechanical-till, stubble-retained plots still lay below the critical N concentration. Zero-till, stubble-retained treatment had least nitrate-N in the soil profile to 120 cm indicating a problem in N supply rather than in N recovery by the roots. The effects of the long-term treatments on properties related to the N supplying capacity of the soil were investigated by determinations on topsoil samples from which undecomposed stubble was removed. Soil from zero-till, stubble-retained treatment had more organic carbon (C) and Kjeldahl N than mechanical-till, stubble-retained treatment which had more than stubble-burnt treatments. The C: N ratio of the soil was lower in stubble retained treatments. Kjeldahl N was higher with annual N fertilization only where stubble was retained. Organic C increased and Kjeldahl N decreased over a 3 year period in all treatments. Respiration of CO2, mineralizable N, and microbial biomass C and N in the soil were all greater with long-term stubble retention than with stubble burning irrespective of tillage treatment. Numbers of root-lesion nematodes (Pratylenchus thornei Sher and Allen) and stunt nematode (Merlinius brevidens (Allen) Siddiqi) following a wheat crop were substantially greater with zero-till than with mechanical-till. Root-lesion nematode were increased by N fertilization of previous crops while stunt nematodes were increased by stubble-retention. Earthworm numbers were increased by stubble retention particularly when combined with zero tillage. Factors responsible for observed differences in soil nitrate and crop response to N in this field experiment appear to be: (a) N immobilization by recently retained stubble, (b) lower rates of mineralization of soil N under surface-retained stubble, and (c) higher rates of leaching in zero-till treatment.