DEGENERATION OF PHOTORECEPTORS IN RHODOPSIN MUTANTS OF DROSOPHILA

被引:87
作者
LEONARD, DS [1 ]
BOWMAN, VD [1 ]
READY, DF [1 ]
PAK, WL [1 ]
机构
[1] PURDUE UNIV,DEPT BIOL SCI,W LAFAYETTE,IN 47907
来源
JOURNAL OF NEUROBIOLOGY | 1992年 / 23卷 / 06期
关键词
RHODOPSIN MUTANTS; PHOTORECEPTOR DEGENERATION; DROSOPHILA;
D O I
10.1002/neu.480230602
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Five different, well-characterized mutants of the R1-6 rhodopsin gene (ninaE), which corresponds to the rod opsin gene of vertebrates, have been examined morphologically as a function of age (up to 9 weeks) to determine whether or not the photoreceptors degenerate and to assess the pattern of degeneration. Structural deterioration of R1-6 photoreceptors with age has been found in all five mutants. The structural pattern of degeneration is similar in the five mutants, but the time course of degeneration is allele dependent and varies greatly among the five, with the strongest alleles causing the fastest degeneration. The degeneration appears to be independent of either the illumination cycle to which the animals are exposed or the presence of screening pigments in the eye. Although the degeneration first appears in R1-6 photoreceptors, eventually R7/8 photoreceptors, which correspond to cones of vertebrates, are also affected. In many of these mutants. striking proliferations of membrane processes have been observed in the subrhabdomeric region of R1-6 photoreceptors. It is hypothesized that (1) this accumulation of membranes may be caused by the failure of newly synthesized membranes that are inserted into the base of microvilli to be assembled into R1-6 rhabdomeres and (2) this failure may be caused by the extremely low concentration of normal R1-6 rhodopsin in the ninaE mutants.
引用
收藏
页码:605 / 626
页数:22
相关论文
共 42 条
[1]  
BAUMANN O, 1989, CELL TISSUE RES, V255, P511, DOI 10.1007/BF00218786
[2]  
BERSON EL, 1975, ADLERS PHYSL EYE, P453
[4]   A POINT MUTATION OF THE RHODOPSIN GENE IN ONE FORM OF RETINITIS-PIGMENTOSA [J].
DRYJA, TP ;
MCGEE, TL ;
REICHEL, E ;
HAHN, LB ;
COWLEY, GS ;
YANDELL, DW ;
SANDBERG, MA ;
BERSON, EL .
NATURE, 1990, 343 (6256) :364-366
[5]   MUTATION SPECTRUM OF THE RHODOPSIN GENE AMONG PATIENTS WITH AUTOSOMAL DOMINANT RETINITIS-PIGMENTOSA [J].
DRYJA, TP ;
HAHN, LB ;
COWLEY, GS ;
MCGEE, TL ;
BERSON, EL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (20) :9370-9374
[6]  
DUKEELDER S, 1967, DISEASES RETINA
[7]   CHANGES IN RETINAL FINE STRUCTURE INDUCED IN CRAB LIBINIA BY LIGHT AND DARK ADAPTATION [J].
EGUCHI, E ;
WATERMAN, TH .
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE, 1967, 79 (02) :209-+
[8]   HEREDITARY RETINAL DEGENERATION IN DROSOPHILA-MELANOGASTER - MUTANT DEFECT ASSOCIATED WITH PHOTO-TRANSDUCTION PROCESS [J].
HARRIS, WA ;
STARK, WS .
JOURNAL OF GENERAL PHYSIOLOGY, 1977, 69 (03) :261-291
[9]  
HARRIS WA, 1976, J PHYSIOL-LONDON, V256, P415
[10]   ELECTROPHYSIOLOGICAL STUDY OF DROSOPHILA RHODOPSIN MUTANTS [J].
JOHNSON, EC ;
PAK, WL .
JOURNAL OF GENERAL PHYSIOLOGY, 1986, 88 (05) :651-673