SYNTHESIS OF AN AFFINITY ADSORBENT FOR SIALIDASE

被引:9
作者
SUZUKI, J [1 ]
MURAKAMI, K [1 ]
NISHIMURA, Y [1 ]
机构
[1] INST MICROBIAL CHEM,SHINAGAWA KU,TOKYO 141,JAPAN
关键词
D O I
10.1080/07328309308021270
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The reaction of the sodium salt of methyl 5-acetamido-4,7,8,9-tetra-0-acetyl-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-2-nonulopyranosonate (1) with styrene oxide, followed by deprotection, gave the diastereomeric mixture of (beta-hydroxyphenethyl) 5-acetamido-3,5-dideoxy-2-thio-D-glycero-alpha-D-galacto-2-nonulopyranosidoic acid (3). The thioglycoside 3 was proved to be an inhibitor for Clostridium perfringens sialidase. An affinity adsorbent with immobilized sialic acid through a thioglycosidic linkage was prepared by treatment of 1 with epoxy-activated Sepharose 4B. The synthetic adsorbent was proved to adsorb Cl. perfringens sialidase specifically and to be capable of repeated operation.
引用
收藏
页码:201 / 208
页数:8
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