SEPARATION OF CIS AND TRANS UNSATURATED FATTY-ACID METHYL-ESTERS BY SILVER ION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：59

作者：

ADLOF, RO

机构：

[1] US Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 659卷 / 01期

关键词：

D O I：

10.1016/0021-9673(94)85010-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A variety of isomeric fatty acid methyl esters were separated on a commercially available silver ion chromatography column utilizing a versatile, isocratic solvent system composed of acetonitrile in hexane and utilizing UV detection. Samples containing all possible cis and trans isomers derived from methyl oleate (cis-9-oetadecenoate; 18:1), methyl linoleate (cis-9, cis-12-octadecadienoate; 18:2), methyl linolenate (cis-9, cis-12, cis-15-octadecatrienoate; 18:3) and methyl arachidonate (cis-5, cis-8, cis-11, cis-14-eicosatetraenoate; 20:4) were analyzed. The cis and trans isomers from methyl oleate, methyl linoleate and methyl linolenate were resolved, as were 15 of the 16 isomers from methyl arachidonate. The separation of the 20:4 isomers exceeded the capability of capillary gas and liquid chromatographic methodologies.

引用

页码：95 / 99

页数：5

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