EFFECT OF TRICHOPLUSIA-NI BTI-TN 5B1-4 CELL-DENSITY ON HUMAN SECRETED ALKALINE-PHOSPHATASE PRODUCTION

被引：13

作者：

CHUNG, IS ^{[1
]}

SHULER, ML ^{[1
]}

机构：

[1] CORNELL UNIV, SCH CHEM ENGN, ITHACA, NY 14853 USA

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 10期

关键词：

D O I：

10.1007/BF00129927

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of Tn 5B1-4 cell density at infection on the production of human secreted alkaline phosphatase (SEAP) was investigated using a split-flow air-lift bioreactor. Volumetric productivities of SEAP were highest when Tn 5B1-4 cells were infected at about 3 x 10(5) cells/cm2. Cell-to-cell contact inhibition is an important factor responsible for the reduced protein production at high cell densities. Other factors such as oxygen or nutrient limitation, or build up of metabolic inhibitors do not appear to be as important.

引用

收藏

页码：1007 / 1012

页数：6

相关论文

共 15 条

[1] SECRETED PLACENTAL ALKALINE-PHOSPHATASE - A POWERFUL NEW QUANTITATIVE INDICATOR OF GENE-EXPRESSION IN EUKARYOTIC CELLS [J].

BERGER, J ;

HAUBER, J ;

HAUBER, R ;

GEIGER, R ;

CULLEN, BR .

GENE, 1988, 66 (01) :1-10

[2] HIGH-LEVEL RECOMBINANT PROTEIN-PRODUCTION IN BIOREACTORS USING THE BACULOVIRUS INSECT CELL EXPRESSION SYSTEM [J].

CARON, AW ;

ARCHAMBAULT, J ;

MASSIE, B .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 36 (11) :1133-1140

[3]

CHUNG IS, 1993, IN PRESS BIOTECHNOL

[4] BACULOVIRUS EXPRESSION OF ALKALINE-PHOSPHATASE AS A REPORTER GENE FOR EVALUATION OF PRODUCTION, GLYCOSYLATION AND SECRETION [J].

DAVIS, TR ;

TROTTER, KM ;

GRANADOS, RR ;

WOOD, HA .

BIO-TECHNOLOGY, 1992, 10 (10) :1148-1150

[5]

DAVIS TR, 1992, IN PRESS IN VITRO CE

[6] PROPAGATION OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS IN CELL-CULTURE - METHODS FOR INFECTING CELLS [J].

HINK, WF ;

STRAUSS, EM ;

RAMOSKA, WA .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1977, 30 (02) :185-191

[7] OPTIMIZATION OF THE PRODUCTION OF FULL-LENGTH RCD4 IN BACULOVIRUS-INFECTED SF9 CELLS [J].

LAZARTE, JE ;

TOSI, PF ;

NICOLAU, C .

BIOTECHNOLOGY AND BIOENGINEERING, 1992, 40 (02) :214-217

[8] QUANTIFICATION OF CELL-CULTURE FACTORS AFFECTING RECOMBINANT PROTEIN YIELDS IN BACULOVIRUS-INFECTED INSECT CELLS [J].

LINDSAY, DA ;

BETENBAUGH, MJ .

BIOTECHNOLOGY AND BIOENGINEERING, 1992, 39 (06) :614-618

[9] INFLUENCE OF CONDITION OF CELLS AND MEDIUM ON PRODUCTION OF POLYHEDRA OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS INVITRO [J].

STOCKDALE, H ;

GARDINER, GR .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1977, 30 (03) :330-336

[10] PRODUCTION OF NUCLEAR POLYHEDROSIS VIRUSES IN LARGE-VOLUME CELL-CULTURES [J].

VAUGHN, JL .

JOURNAL OF INVERTEBRATE PATHOLOGY, 1976, 28 (02) :233-237