DELETION OF DNA ENCODING THE 1ST 5 TRANSMEMBRANE DOMAINS OF EPSTEIN-BARR-VIRUS LATENT MEMBRANE PROTEIN-2A AND PROTEIN-2B

被引:87
作者
LONGNECKER, R
MILLER, CL
TOMKINSON, B
MIAO, XQ
KIEFF, E
机构
[1] HARVARD UNIV,SCH MED,DEPT MED,BOSTON,MA 02115
[2] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,BOSTON,MA 02115
关键词
D O I
10.1128/JVI.67.8.5068-5074.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A recombinant Epstein-Barr virus (EBV) was constructed, with a positive-selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte growth transformation in vitro. Cells transformed with the mutant recombinant were not different from wild-type virus transformants in initial or long-term outgrowth, sensitivity to limiting cell dilution, or serum requirement. Expression of EBNA1, EBNA2, EBNA3A, EBNA3C, and LMP1 and permissivity for lytic EBV infection were also unaffected by the LMP2 deletion mutation. These results complete the molecular genetic studies proving LMP2 is dispensable for primary B-lymphocyte growth transformation, latent infection, and lytic virus replication in vitro.
引用
收藏
页码:5068 / 5074
页数:7
相关论文
共 38 条
[1]  
BAICHWAL VR, 1988, ONCOGENE, V2, P461
[2]   EPSTEIN-BARR-VIRUS LATENT GENE-TRANSCRIPTION IN NASOPHARYNGEAL CARCINOMA-CELLS - COEXPRESSION OF EBNA1, LMP1, AND LMP2 TRANSCRIPTS [J].
BROOKS, L ;
YAO, QY ;
RICKINSON, AB ;
YOUNG, LS .
JOURNAL OF VIROLOGY, 1992, 66 (05) :2689-2697
[3]   AN EPSTEIN-BARR-VIRUS TRANSFORMATION-ASSOCIATED MEMBRANE-PROTEIN INTERACTS WITH SRC FAMILY TYROSINE KINASES [J].
BURKHARDT, AL ;
BOLEN, JB ;
KIEFF, E ;
LONGNECKER, R .
JOURNAL OF VIROLOGY, 1992, 66 (08) :5161-5167
[4]   CONSISTENT TRANSCRIPTION OF THE EPSTEIN-BARR-VIRUS LMP2 GENE IN NASOPHARYNGEAL CARCINOMA [J].
BUSSON, P ;
MCCOY, R ;
SADLER, R ;
GILLIGAN, K ;
TURSZ, T ;
RAABTRAUB, N .
JOURNAL OF VIROLOGY, 1992, 66 (05) :3257-3262
[5]   POLYMORPHIC PROTEINS ENCODED WITHIN BZLF1 OF DEFECTIVE AND STANDARD EPSTEIN-BARR VIRUSES DISRUPT LATENCY [J].
COUNTRYMAN, J ;
JENSON, H ;
SEIBL, R ;
WOLF, H ;
MILLER, G .
JOURNAL OF VIROLOGY, 1987, 61 (12) :3672-3679
[6]   IDENTIFICATION OF EPSTEIN-BARR-VIRUS TERMINAL PROTEIN-1 (TP1) IN EXTRACTS OF 4 LYMPHOID-CELL LINES, EXPRESSION IN INSECT CELLS, AND DETECTION OF ANTIBODIES IN HUMAN SERA [J].
FRECH, B ;
ZIMBERSTROBL, U ;
SUENTZENICH, KO ;
PAVLISH, O ;
LENOIR, GM ;
BORNKAMM, GW ;
MUELLERLANTZSCH, N .
JOURNAL OF VIROLOGY, 1990, 64 (06) :2759-2767
[7]   TRANSFECTION OF A REARRANGED VIRAL-DNA FRAGMENT, WZHET, STABLY CONVERTS LATENT EPSTEIN-BARR VIRAL-INFECTION TO PRODUCTIVE INFECTION IN LYMPHOID-CELLS [J].
GROGAN, E ;
JENSON, H ;
COUNTRYMAN, J ;
HESTON, L ;
GRADOVILLE, L ;
MILLER, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (05) :1332-1336
[8]   EPSTEIN-BARR-VIRUS LATENT MEMBRANE-PROTEIN EXPRESSION IN HODGKIN AND REED-STERNBERG CELLS [J].
HERBST, H ;
DALLENBACH, F ;
HUMMEL, M ;
NIEDOBITEK, G ;
PILERI, S ;
MULLERLANTZSCH, N ;
STEIN, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (11) :4766-4770
[9]  
KAYE KM, UNPUB EPSTEIN BARR V
[10]  
KIEFF E, 1990, VIROLOGY, P1889