NERVE GROWTH FACTOR-MEDIATED INDUCTION OF TYROSINE-HYDROXYLASE IN A CLONAL PHEOCHROMOCYTOMA CELL LINE

被引：47

作者：

GOODMAN, R ^{[1
]}

HERSCHMAN, HR ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,SCH MED,NUCL MED & RADIAT BIOL LAB,LOS ANGELES,CA 90024

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1978年 / 75卷 / 09期

关键词：

D O I：

10.1073/pnas.75.9.4587

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The authors have established a clonal cell line, PC-G2, from an experimentally induced rat pheochromocytoma. Administration of nerve growth factor to PC-G2 causes a 4- to 8-fold induction in the specific activity of tyrosine hydroxylase [tyrosine 3-monooxygenase; L-tyrosine, tetrahydropteridine:oxygen oxidoreductase (3-hydroxylating); EC 1.14.16.2]. The response is elicited in a dose-dependent fashion, at concentrations above 0.1 μg/ml. Antiserum to nerve growth factor inhibited the induction of tyrosine hydroxylase. Dexamethasone enhances the nerve growth factor-mediated elevation tyrosine hydroxylase. After 3-4 days of exposure to nerve growth factor the maximal induction of tyrosine hydroxylase is seen, although a significant increase can be observed after 24 hr. In contrast to the PC-12 cell line (derived from the same tumor), in which neurite outgrowth occurs in response to nerve growth factor, there is no morphological change or alteration in growth rate of PC-G2 cells after exposure to nerve growth factor.

引用

页码：4587 / 4590

页数：4

共 23 条

[1] NERVE GROWTH-FACTOR [J].

BRADSHAW, RA .

ANNUAL REVIEW OF BIOCHEMISTRY, 1978, 47 :191-216

[2] SELECTION FOR NEUROBLASTOMA-CELLS THAT SYNTHESIZE CERTAIN TRANSMITTERS [J].

BREAKEFIELD, XO ;

NIRENBERG, MW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (06) :2530-2533

[3] ROLE OF NERVE GROWTH-FACTOR IN DEVELOPMENT OF RAT SYMPATHETIC NEURONS INVITRO .1. SURVIVAL, GROWTH, AND DIFFERENTIATION OF CATECHOLAMINE PRODUCTION [J].

CHUN, LLY ;

PATTERSON, PH .

JOURNAL OF CELL BIOLOGY, 1977, 75 (03) :694-704

[4] EMBRYOLOGIC DEVELOPMENT OF A MOUSE SYMPATHETIC-GANGLION INVIVO AND INVITRO [J].

COUGHLIN, MD ;

BOYER, DM ;

BLACK, IB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3438-3442

[5] GLUCOCORTICOID INDUCTION OF TYROSINE-HYDROXYLASE IN A CONTINUOUS CELL LINE OF RAT PHEOCHROMOCYTOMA [J].

GOODMAN, R ;

EDGAR, D ;

THOENEN, H ;

WECHSLER, W ;

HERSCHMAN, H .

JOURNAL OF CELL BIOLOGY, 1978, 78 (01) :R1-R7

[6] ESTABLISHMENT OF A NORADRENERGIC CLONAL LINE OF RAT ADRENAL PHEOCHROMOCYTOMA CELLS WHICH RESPOND TO NERVE GROWTH-FACTOR [J].

GREENE, LA ;

TISCHLER, AS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (07) :2424-2428

[7]

GREENE LA, 1974, NEUROBIOLOGY, V4, P286

[8]

LEVIMONT.R, 1966, PHARMACOL REV, V18, P619

[9] NERVE GROWTH-FACTOR INDUCES VOLUME INCREASE AND ENHANCES TYROSINE-HYDROXYLASE SYNTHESIS IN CHEMICALLY AXOTOMIZED SYMPATHETIC-GANGLIA OF NEWBORN RATS [J].

LEVIMONTALCINI, R ;

ALOE, L ;

MUGNAINI, E ;

OESCH, F ;

THOENEN, H .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (02) :595-599

[10] ESSENTIAL ROLE OF NERVE GROWTH FACTOR IN SURVIVAL AND MAINTENANCE OF DISSOCIATED SENSORY AND SYMPATHETIC EMBRYONIC NERVE CELLS IN VITRO [J].

LEVIMONTALCINI, R ;

ANGELETTI, PU .

DEVELOPMENTAL BIOLOGY, 1963, 7 (01) :653-&

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