ETHIDIUM DIMER - NEW REAGENT FOR THE FLUORIMETRIC DETERMINATION OF NUCLEIC-ACIDS

被引：106

作者：

MARKOVITS, J ^{[1
]}

ROQUES, BP ^{[1
]}

LEPECQ, JB ^{[1
]}

机构：

[1] UER SCI PHARMACEUT & BIOL,CNRS,EQUIPE RECH 613,DEPT CHIM ORGAN,F-75270 PARIS 06,FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90357-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A dimeric derivative of ethidium is used for fluorimetric assay of nucleic acids. Due to the very high binding affinity of this derivative for DNA and RNA, a significant increase of sensitivity of the ethidium fluorimetric technique for nucleic acids determination is obtained. Using a commercially available instrument, DNA concentrations in the nanogram per milliliter range are determined. In addition, we have found that an acridine ethidium dimer can also be used for a sensitive fluorimetric assay of DNA concentration, while simultaneously providing a determination of the DNA base composition. © 1979.

引用

页码：259 / 264

页数：6

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