INVITRO SYNTHESIS OF CHLOROPLAST FERREDOXIN AS A HIGH MOLECULAR-WEIGHT PRECURSOR IN A CELL-FREE PROTEIN SYNTHESIZING SYSTEM FROM WHEAT GERMS

被引：47

作者：

HUISMAN, JG ^{[1
]}

MOORMAN, AFM ^{[1
]}

VERKLEY, FN ^{[1
]}

机构：

[1] UNIV AMSTERDAM,MED ENZYMOL & MOLEC BIOL SECT,BIOCHEM LAB,AMSTERDAM 1004,NETHERLANDS

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1978年 / 82卷 / 04期

关键词：

D O I：

10.1016/0006-291X(78)90303-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA isolated from leaves of Nicotiana tabacum, etiolated leaves of Phaseolus vulgaris and from cells of Chlamydomonas reinhardii, can be translated in cell-free extracts derived from wheat germs. The in vitro products were identified by SDS electrophoresis after immunoprecipitation with specific antisera against ferredoxin. The in vitro products appeared to have a Mr 20,500, in contrast to authentic ferredoxin that has a Mr 17,000. We propose that chloroplast ferredoxin is synthesized in vitro as a biosynthetic precursor. Ferredoxin synthesis was found to be associated with poly(A)-RNA, which supports our previous conclusion that ferredoxin messenger is a transcript from the DNA of the nucleus. © 1978.

引用

页码：1121 / 1131

页数：11

共 30 条

[1] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[2] DIFFERENTIAL RECOGNITION OF CHLOROPLAST AND CYTOPLASMIC MESSENGER-RNA BY 70S AND 80S RIBOSOMAL SYSTEMS [J].

BOTTOMLEY, W ;

HIGGINS, TJV ;

WHITFELD, PR .

FEBS LETTERS, 1976, 63 (01) :120-124

[3] BIOCHEMISTRY OF PROTEIN-SYNTHESIS IN PLANTS [J].

BOULTER, D ;

ELLIS, RJ ;

YARWOOD, A .

BIOLOGICAL REVIEWS, 1972, 47 (01) :113-+

[4]

BUCHANAN BB, 1970, ADV ENZYMOL REL S BI, V33, P119

[5] TRANSLATION OF VIRUS MESSENGER-RNA - SYNTHESIS OF BACTERIOPHAGE-Q-BETA PROTEINS IN A CELL-FREE EXTRACT FROM WHEAT EMBRYO [J].

DAVIES, JW ;

KAESBERG, P .

JOURNAL OF VIROLOGY, 1973, 12 (06) :1434-1441

[6] INVITRO SYNTHESIS AND PROCESSING OF A PUTATIVE PRECURSOR FOR SMALL SUBUNIT OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE OF CHLAMYDOMONAS-REINHARDTII [J].

DOBBERSTEIN, B ;

BLOBEL, G ;

CHUA, NH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (03) :1082-1085

[7] YEAST MITOCHONDRIAL RNA DOES NOT CONTAIN POLY(A) [J].

GROOT, GSP ;

FLAVELL, RA ;

VANOMMEN, GJ ;

GRIVELL, LA .

NATURE, 1974, 252 (5479) :167-169

[8] SYNTHESIS AND TRANSPORT OF SMALL SUBUNIT OF CHLOROPLAST RIBULOSE BISPHOSPHATE CARBOXYLASE [J].

HIGHFIELD, PE ;

ELLIS, RJ .

NATURE, 1978, 271 (5644) :420-424

[9] 2 DIFFERENT PLANT-TYPE FERREDOXINS IN EACH OF 2 PETUNIA SPECIES [J].

HUISMAN, JG ;

STAPEL, S ;

MUIJSERS, AO .

FEBS LETTERS, 1978, 85 (02) :198-202

[10] CODING SITE OF CHLOROPLAST FERREDOXIN [J].

HUISMAN, JG ;

GEBBINK, MGT ;

MODDERMAN, P ;

STEGWEE, D .

PLANTA, 1977, 137 (02) :97-105

← 1 2 3 →