CALLUS FORMATION FROM CELL-CULTURE PROTOPLASTS OF CORN (ZEA-MAYS-L)

被引:104
作者
POTRYKUS, I
HARMS, CT
LORZ, H
机构
[1] Friedrich Miescher Institut, Basel
关键词
Callus formation; Protoplasts; Suspension culture; Zea mays L;
D O I
10.1007/BF00267709
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Routine procedures for the isolation of large numbers of protoplasts from an established cell culture of Zea mays and for the induction of sustained divisions leading to secondary cell cultures have been developed. The critical factors seem to be associated with neither specific enzymatic conditions for the isolation nor specific culture conditions for the protoplasts but with the 'quality' of the culture used for protoplast isolation. © 1979 Springer-Verlag.
引用
收藏
页码:209 / 214
页数:6
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