SANDWICH ENZYME-IMMUNOASSAY FOR RAT TRANSFERRIN WITH 2 MONOCLONAL-ANTIBODIES AND ITS APPLICATION

被引：7

作者：

MATSUMOTO, T

SHIMA, H

KISHI, T

SATO, T

机构：

[1] Department of Biochemistry, Research Laboratory of Applied Biochemistry, Tanabe Seiyaku Company Ltd., Yodogawa-ku, Osaka, 532, 16-89

来源：

BIOCHEMICAL MEDICINE AND METABOLIC BIOLOGY | 1991年 / 45卷 / 02期

关键词：

D O I：

10.1016/0885-4505(91)90020-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of a sandwich enzyme immunoassay for rat transferrin with two monoclonal antibodies is described. Microtiter plates coated with one monoclonal antibody (15C2H3) were used, and captured transferrin was estimated with a horseradish peroxidase-conjugated Fab' fragment of another monoclonal antibody (22A06D2). In this assay, the measurable range is 5-150 ng/ml and the coefficients of variation within and between the assay series are 1.2-5.0 and 3.3-6.0%, respectively. Recovery was 101 ± 9.7% when purified rat transferrin was added to rat plasma. No cross-reactivity with bovine, human, or mouse transferrin was shown. This assay for rat transferrin is a highly specific, sensitive, and expeditious method which may allow routine analysis of rat transferrin in blood or culture supernatants of rat hepatocytes. © 1991.

引用

页码：188 / 196

页数：9

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