USE OF ESCHERICHIA-COLI CLONED WITH GENES ENCODING BACTERIAL LUCIFERASE FOR EVALUATION OF CHEMICAL TOXICITY

被引:16
作者
LAMPINEN, J
KORPELA, M
SAVIRANTA, P
KRONELD, R
KARP, M
机构
[1] Department of Biochemistry, University of Turku, Turku
[2] Bio‐Orbit Oy, Turku, SF-20750
[3] Åbolands Sjukhus, Turku, SF-20700
来源
TOXICITY ASSESSMENT | 1990年 / 5卷 / 04期
关键词
D O I
10.1002/tox.2540050403
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
A method for evaluation of toxicity of aqueous solutions is described based on the use of genetically engineered Escherichia coli. The genes encoding bacterial luciferase have been cloned from Vibrio harveyi to a deep rough mutant of E. coli under the control of the lac promoter. Light production by this strain has been stabilized by optimizing several parameters having an effect on the gene expression. Toxicity measurements were performed for selected metals and organic solvents to determine the sensitivity of the test strain. Effective concentrations calculated from these measurements show that this method has a sensitivity equal to other normally used methods. The test can be performed using buffers with low ionic strength without any significant change in the stability of the light emitted. Moreover, the method does not necessitate the use of special equipment or skills. Copyright © 1990 Wiley Periodicals, Inc., A Wiley Company
引用
收藏
页码:337 / 350
页数:14
相关论文
共 16 条
[1]  
Benford D.J., Hubbard S.A., Preparature and Culture of Mammalian Cells, Biochemical Toxicolog, pp. 57-82, (1987)
[2]  
Brock T.D., Smith D.W., Madigan M.T., Biology of Microorganism, (1979)
[3]  
Bulich A.A., Greene M.W., Use of Luminescent Bacteria for Biological Monitoring of Water Qualit, pp. 193-211, (1979)
[4]  
Bulich A.A., A practical reliable method for monitoring the toxicity of aquatic samples, Proc. Biochem. March/April, 17, pp. 45-47, (1982)
[5]  
Gadd G.M., Griffiths A.I., Microorganisms and heavy metal toxicity, Microb. Ecol., 4, pp. 303-317, (1978)
[6]  
Gupta S.G., Reese C.P., Hastings J.W., Mobilization of cloned luciferase genes in Vibrio harveyi luminescence mutants, Arch. Microbiol., 143, pp. 325-329, (1985)
[7]  
Hastings J.W., Balny C., The oxygenated bacterial luciferase‐flavin intermediate, reaction products via the light and dark pathways, J. Biol. Chem., 250, pp. 7288-7293, (1975)
[8]  
Hastings J.W., Bacterial Bioluminescence: an Overview, Methods in Enzymology, 57, pp. 125-135, (1978)
[9]  
Hinwood A.L., McCormick M.J., The effect of ionic solutes on EC<sub>50</sub> values measured using the Microtox test, Toxic. Assess., 2, pp. 449-461, (1987)
[10]  
Korpela M., Karp M., Stable‐light producing Escherichia coli, Biotechnol. Lett., 10, pp. 383-388, (1988)