ELECTROPHORETIC KARYOTYPING AND CHROMOSOMAL GENE-MAPPING OF CHLORELLA

被引:41
作者
HIGASHIYAMA, T [1 ]
YAMADA, T [1 ]
机构
[1] HIROSHIMA UNIV,FAC ENGN,1-4-1 KAGAMIYAMA,HIROSHIMA 724,JAPAN
关键词
D O I
10.1093/nar/19.22.6191
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Molecular karyotypes for six strains of four Chlorella species were obtained by using an alternating-field gel electrophoresis system which employs contour-clamped homogenous electric fields (CHEF). The number and migration pattern of the chromosomal DNA molecules varied greatly, from strain to strain: for example, nine separated chromosomes of C. ellipsoidea C87 ranged from 2.5 to 6.5 megabase pairs (mbp) in size, whereas 16 chromosomes of C. vulgaris C169 were from 980 kilobase pairs (kbp) to 4.0 mbp. Depending on the chromosome migration patterns, the six strains were classified into two major chromosome-length polymorphism groups. Using hybridization techniques, the genes for alpha-tublin, chlorophyll-a, b-binding proteins, ribosomal RNAs, and the small subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO) were mapped on the separated chromosomes of C. vulgaris C169. Since Chlorella chromosomes are small enough to separate and isolate individually by CHEF gel electrophoresis under ordinary conditions, they should serve as excellent materials to study the fundamental molecular structure of plant-type chromosomes.
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页码:6191 / 6195
页数:5
相关论文
共 24 条
[1]  
ALLEN MB, 1971, METHOD ENZYMOL, V23, P168
[2]   ELECTROPHORETIC KARYOTYPE OF ASPERGILLUS-NIDULANS [J].
BRODY, H ;
CARBON, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (16) :6260-6263
[3]   SEPARATION OF LARGE DNA-MOLECULES BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS [J].
CHU, G ;
VOLLRATH, D ;
DAVIS, RW .
SCIENCE, 1986, 234 (4783) :1582-1585
[4]   GIARDIA-LAMBLIA - HAPLOID GENOME SIZE DETERMINED BY PULSED FIELD GEL-ELECTROPHORESIS IS LESS THAN 12-MB [J].
FAN, JB ;
KORMAN, SH ;
CANTOR, CR ;
SMITH, CL .
NUCLEIC ACIDS RESEARCH, 1991, 19 (08) :1905-1908
[5]   CONSTRUCTION OF A NOT-I RESTRICTION MAP OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE GENOME [J].
FAN, JB ;
CHIKASHIGE, Y ;
SMITH, CL ;
NIWA, O ;
YANAGIDA, M ;
CANTOR, CR .
NUCLEIC ACIDS RESEARCH, 1989, 17 (07) :2801-2818
[6]  
Fott B., 1969, STUD PHYCO, P10
[7]   SEQUENCE, EVOLUTION AND DIFFERENTIAL EXPRESSION OF THE 2 GENES ENCODING VARIANT SMALL SUBUNITS OF RIBULOSE BISPHOSPHATE CARBOXYLASE-OXYGENASE IN CHLAMYDOMONAS-REINHARDTII [J].
GOLDSCHMIDTCLERMONT, M ;
RAHIRE, M .
JOURNAL OF MOLECULAR BIOLOGY, 1986, 191 (03) :421-432
[8]  
Govindjee, 1974, ALGAL PHYSL BIOCH, P346
[9]   KARYOTYPING OF YEAST STRAINS OF SEVERAL GENERA BY FIELD INVERSION GEL-ELECTROPHORESIS [J].
JOHNSTON, JR ;
CONTOPOULOU, CR ;
MORTIMER, RK .
YEAST, 1988, 4 (03) :191-198
[10]   SIZE VARIATION IN CHROMOSOMES FROM INDEPENDENT CULTURED ISOLATES OF PLASMODIUM-FALCIPARUM [J].
KEMP, DJ ;
CORCORAN, LM ;
COPPEL, RL ;
STAHL, HD ;
BIANCO, AE ;
BROWN, GV ;
ANDERS, RF .
NATURE, 1985, 315 (6017) :347-350