DEGRADATION OF PLASMA-PROTEINS BY THE TRYPSIN-LIKE-ENZYME OF PORPHYROMONAS-GINGIVALIS AND INHIBITION OF PROTEASE ACTIVITY BY A SERINE PROTEASE INHIBITOR OF HUMAN PLASMA

被引：34

作者：

FISHBURN, CS ^{[1
]}

SLANEY, JM ^{[1
]}

CARMAN, RJ ^{[1
]}

CURTIS, MA ^{[1
]}

机构：

[1] LONDON HOSP,COLL MED,MRC,DENT RES UNIT,32 NEWARK ST,LONDON E1 2AA,ENGLAND

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 1991年 / 6卷 / 04期

关键词：

PORPHYROMONAS-GINGIVALIS; TRYPSIN-LIKE ENZYME; PLASMA PROTEOLYSIS; PROTEASE INHIBITOR;

D O I：

10.1111/j.1399-302X.1991.tb00479.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The interaction between Porphyromonas gingivalis culture supernatant and human serum was examined. Hydrolysis of the major serum proteins was thiol-dependent and correlated with the trypsin-like activity of the sample. Transferrin and IgG light chains were less susceptible to degradation than albumin and IgG heavy chains and partially degraded IgG retained antigen-binding capability. Serum inhibited the trypsin-like activity in a fluorimetric assay. The inhibition was shown to be independent of the level of IgG antibody reactive with whole cells of P. gingivalis. Purified preparations of antithrombin III, a serine protease inhibitor, but not alpha-1-antitrypsin nor alpha-2-macroglobulin inhibited the trypsin-like activity in the fluorimetric assay.

引用

页码：209 / 215

页数：7

共 26 条

[1] Carlone GM, Thomas ML, Rumschlag HS, Sottnek FO, Rapid microprocedure for isolating detergent insoluble outer membrane proteins from Haemophilus species, J Clin Microbiol, 2, pp. 293-296, (1986)
[2] Carlsson J, Herrman BF, Hoflung JF, Sundqvist GK, Degradation of the human proteinase inhibitors alpha‐1 anti‐trypsin and alpha‐2 macroglobulin by Bacteroides gingivalis, Infect Immun, 43, pp. 644-648, (1984)
[3] Carlsson J, Hoflung JG, Sundvist GK, Degradation of albumin, haemopexin, haptoglobin and transferrin by black‐pigmented Bacteroides species, J Med Microbiol, 18, pp. 39-46, (1984)
[4] Carman RJ, Ramakrishnan MD, Harper FH, Curtis MA, Bacteroides gingivalis: role of haemin and iron in aetiology of destructive periodontitis, Anaerobes in medicine and industry
[5] Carrell RW, Pemberton PA, Boswell DR, The serpins: evolution and adaptation in a family of protease inhibitors, Cold Spring Harbour Symposia Quantitative Biol, 52, pp. 527-535, (1987)
[6] Curtis MA, Slaney JM, Carman RJ, Et al.
[7] Cutress TW, Ainamo J, Sardo-Infirri J, The Community Periodontal Index of Treatment Needs (CPITN): procedure for population groups and individuals, Int Dent J, 37, pp. 222-233, (1987)
[8] Ebersole JL, Taubman MA, Smith DJ, Frey DE, Human immune responses to oral microorganisms: patterns of systemic antibody levels to Bacteroides species, Infect Immun, 51, pp. 507-513, (1986)
[9] Genco RJ, Slots J, Host responses in periodontal diseases, J Dent Res, 63, pp. 441-451, (1984)
[10] Grenier D, Mayrand D, McBride BC, Further studies on the degradation of immunoglobulins by black‐pigmented Bacteroides, Oral Microbiol Immunol, 4, pp. 12-18, (1989)

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