BASE SEQUENCES OF HIGHLY REPETITIVE COMPONENTS IN NUCLEAR DNAS FROM RAT-LIVER AND RAT-ASCITES HEPATOMA

被引：14

作者：

IKEDA, Y ^{[1
]}

NAKAMURA, K ^{[1
]}

IWAKAMI, N ^{[1
]}

HIBINO, Y ^{[1
]}

SUGANO, N ^{[1
]}

机构：

[1] TOYAMA MED & PHARMACEUT UNIV,FAC PHARMACEUT SCI,DIV CELL BIOL,SUGITANI,TOYAMA 93001,JAPAN

来源：

CANCER LETTERS | 1990年 / 55卷 / 03期

关键词：

ASCITES HEPATOMA; RAT; HIGHLY REPETITIVE DNA; SEQUENCE;

D O I：

10.1016/0304-3835(90)90120-M

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A 370-bp highly repetitive component in each of the nuclear DNAs from rat liver (RL) and rat-ascites hepatoma (AH) was isolated by HindIII digestion and cloned in pUC9. Ten of the resulting clones were arbitrarily selected and sequenced. Heterogeneity of size was found in 7 of the RL clones (366-369 bp), but in only 2 of the AH clones (369 bp). The sequence homology was 64.6% among the RL clones; 80.3% among the AH clones. The base compositions were AT-rich, ranging from 61.1% to 64.7%. Many A and/or T runs consisting of 2-5' bases were interspersed throughout each sequence. The restriction sites reported previously [12], EcoRI, HaeIII, HindIII, HinfI and HphI sites, were confirmed in almost all of the clones. In the present experiment, 12 kinds of the sites were further found in both RL and AH clones.

引用

页码：201 / 208

页数：8

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