SECRETAGOGUE-INDUCED PROTEOLYSIS OF LUNG SPECTRIN IN ALVEOLAR EPITHELIAL TYPE-II CELLS

被引：15

作者：

ZIMMERMAN, UJP ^{[1
]}

SPEICHER, DW ^{[1
]}

FISHER, AB ^{[1
]}

机构：

[1] UNIV PENN,WISTAR INST,PHILADELPHIA,PA 19104

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1137卷 / 02期

关键词：

CALPAIN; ALVEOLAR EPITHELIAL TYPE-II CELL; SPECTRIN;

D O I：

10.1016/0167-4889(92)90193-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of isolated rat alveolar epithelial type II cells with secretagogues (calcium ionophore, ATP or terbutaline) resulted in rapid proteolysis of lung spectrin and appearance of multiple proteolytic products which showed immunoreactivity with an antibody against human erythrocyte spectrin. These proteolytic products were similar to those generated from erythrocyte spectrin or cultured lung tumor cells (A549 cells) incubated with purified calpain. Furthermore, incubation of alveolar type II cells with a calpain-specific inhibitor modulated the secretagogue-induced proteolysis of lung spectrin. Thus, stimulation of secretion appeared to activate endogenous calpain in type II cells, suggesting that calpain-mediated proteolysis of a submembranous cytoskeletal protein could play an important role in the secretory process.

引用

页码：127 / 134

页数：8

共 37 条

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