GENE REPLACEMENT AND ECTOPIC INTEGRATION IN THE ZYGOMYCETE MUCOR-CIRCINELLOIDES

被引：26

作者：

ARNAU, J

STROMAN, P

机构：

[1] CHR HANSENS LAB DANMARK A-S,DEPT GENET,DK-2970 HORSHOLM,DENMARK

[2] DEPT PHYSIOL,CARLSBERG LAB,DK-2500 VALBY,DENMARK

来源：

CURRENT GENETICS | 1993年 / 23卷 / 5-6期

关键词：

ZYGOMYCETES; TRANSFORMATION; GENE REPLACEMENT; HOMOLOGOUS AND HETEROLOGOUS INTEGRATION;

D O I：

10.1007/BF00312649

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Transformation of Mucor circinelloides with plasmid pTL42 led to the identification of different integrative events. Analysis of eight transformed strains allowed the detection of three classes of transformants: integrative types derived from either homologous (exclusively at the leuA locus) or heterologous recombination and a class which originated by gene replacement. No autonomous replication of the vector was observed.

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页码：542 / 546

页数：5

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