SUPPLEMENTAL COMPLEMENT COMPONENT C9 ENHANCES THE CAPACITY OF NEONATAL SERUM TO KILL MULTIPLE ISOLATES OF PATHOGENIC ESCHERICHIA-COLI

被引:15
作者
LASSITER, HA
WILSON, JL
FELDHOFF, RC
HOFFPAUIR, JM
KLUEBER, KM
机构
[1] UNIV LOUISVILLE, SCH MED, DEPT ANAT SCI & NEUROBIOL, LOUISVILLE, KY 40292 USA
[2] UNIV LOUISVILLE, SCH MED, DEPT BIOCHEM, LOUISVILLE, KY 40292 USA
关键词
D O I
10.1203/00006450-199404000-00002
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Previous studies demonstrated that, compared with adult serum, neonatal serum contained a diminished concentration of complement component C9 and that supplemental C9 enhanced the capacity of neonatal serum to kill an isolate of Escherichia coli. Therefore, experiments were designed to determine the mechanisms by which supplemental C9 enhances the bactericidal capacity of neonatal serum and to determine whether supplemental C9 enhances the capacity of neonatal serum to kill several different pathogenic strains of E. coli. A radiobinding assay and immunogold electron microscopy using a monoclonal anti-C9 antibody revealed that, compared with 40% adult serum, neonatal serum deposited a diminished quantity of C9 onto E. coli O7w:K1:NM. Supplemental C9 (75 mg/L) significantly enhanced the quantity of C9 deposited by the neonatal serum. Treatment with 10 mM MgEGTA (a mixture of 100 mM MgCl2 and 100 mM EGTA that blocks activation of the classic complement pathway but leaves the alternative pathway intact) abolished the capacity of neonatal serum to deposit C9 and to kill the bacteria. Supplemental C9 enhanced the capacity of neonatal serum to kill eight different blood isolates of E. coli. Therefore, supplemental C9 enhanced the capacity of neonatal serum to kill E. coli by increasing the total quantity of C9 deposited via activation of the classic complement pathway. Neonatal serum contained sufficient quantities of classic pathway components, other than C9, to deposit the supplemental C9 onto E. coli and to enhance bacterial killing. The bactericidal activity of neonatal serum against multiple isolates of pathogenic E, coli was increased after C9 supplementation. We speculate that C9 deficiency may be one of the defects in antibacterial host defense that predisposes neonates to the acquisition of E. coli sepsis.
引用
收藏
页码:389 / 396
页数:8
相关论文
共 41 条
[1]  
BALLOW M, 1974, CLIN EXP IMMUNOL, V18, P257
[2]   FORMATION OF TRANSMURAL COMPLEMENT PORES IN SERUM-SENSITIVE ESCHERICHIA-COLI [J].
BHAKDI, S ;
KULLER, G ;
MUHLY, M ;
FROMM, S ;
SEIBERT, G ;
PARRISIUS, J .
INFECTION AND IMMUNITY, 1987, 55 (01) :206-210
[3]  
BIESECKER G, 1980, J IMMUNOL, V124, P1291
[4]   BLOOD AND MARROW NEUTROPHILS DURING EXPERIMENTAL GROUP-B STREPTOCOCCAL INFECTION - QUANTIFICATION OF THE STEM-CELL, PROLIFERATIVE, STORAGE AND CIRCULATING POOLS [J].
CHRISTENSEN, RD ;
MACFARLANE, JL ;
TAYLOR, NL ;
HILL, HR ;
ROTHSTEIN, G .
PEDIATRIC RESEARCH, 1982, 16 (07) :549-553
[5]   FUNCTION OF CLASSICAL AND ALTERNATE PATHWAYS OF HUMAN COMPLEMENT IN SERUM TREATED WITH ETHYLENE-GLYCOL TETRAACETIC ACID AND MGCL2-ETHYLENE GLYCOL TETRAACETIC ACID [J].
DESPREZ, RM ;
BRYAN, CS ;
HAWIGER, J ;
COLLEY, DG .
INFECTION AND IMMUNITY, 1975, 11 (06) :1235-1243
[6]  
FIERER J, 1972, J IMMUNOL, V109, P1156
[7]  
GALLANT AR, 1987, NONLINEAR STATISTICA
[8]  
GITLIN D, 1963, PEDIATRICS, V31, P197
[9]   A 10-YEAR REVIEW OF NEONATAL SEPSIS AND COMPARISON WITH THE PREVIOUS 50-YEAR EXPERIENCE [J].
GLADSTONE, IM ;
EHRENKRANZ, RA ;
EDBERG, SC ;
BALTIMORE, RS .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1990, 9 (11) :819-825
[10]   REACTION-MECHANISMS OF NASCENT C567BAR (REACTIVE LYSIS) .2. KILLING OF A ROUGH FORM OF ESCHERICHIA-COLI BY C567BAR, C8, AND C9 [J].
GOLDMAN, JN ;
AUSTEN, KF .
JOURNAL OF INFECTIOUS DISEASES, 1974, 129 (04) :444-450