EFFECT OF SEVERAL RATES OF FREEZING AND THAWING ON VIABILITY OF MYCOBACTERIUM LEPRAE

被引：7

作者：

LEVY, L

机构：

[1] Public Health Service Hospital, San Francisco

来源：

CRYOBIOLOGY | 1969年 / 6卷 / 01期

关键词：

D O I：

10.1016/S0011-2240(69)80007-6

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The influence of the viability of the organisms of various rates of freezing and thawing of suspensions of M. leprae in 10% glycerol and 0.1% bovine serum albumin was investigated, employing Shepard's mouse footpad technique as a measure of viability. Freezing and thawing produced some loss of viability. Rapid freezing was less deleterious than slow freezing, and approximately 75% of the organisms survived rapid cooling to -60°C and overnight storage at this temperature, followed by slow warming. Study of the additional effect of prolonged storage on the viability of M. leprae is planned. © 1970.

引用

页码：42 / +

页数：1

共 8 条

[1] PHYSICAL FACTORS IMPLICATED IN THE DEATH OF MICRO-ORGANISMS AT SUBZERO TEMPERATURES [J].