MODE OF FORMATION OF 1,6-DIHYDRO-NAD IN NADH PREPARATIONS

被引：13

作者：

GODTFREDSEN, SE ^{[1
]}

OTTESEN, M ^{[1
]}

ANDERSEN, NR ^{[1
]}

机构：

[1] LEO PHARMACEUT PROD, DK-2750 COPENHAGEN, DENMARK

来源：

CARLSBERG RESEARCH COMMUNICATIONS | 1979年 / 44卷 / 02期

关键词：

!sup]13[!/sup]C NMR; carbon-13 nuclear magnetic resonance; high pressure liquid chromatography; HPLC; Lactate dehydrogenase inhibitor; NAD oxidoreductase;

D O I：

10.1007/BF02906522

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The identity of the product obtained by sodium borohydride reduction of NAD+ as 1,6-dihydro-NAD has been verified by13C NMR spectroscopy. The behaviour of this compound during chromatography on Sephadex G-15 has been found identical with that of the humidity induced inhibitor detected in NADH preparations and postulated to be identical with 1,6-dihydro-NAD. Evidence is presented that this inhibitor is responsible for the bulk of the inhibitory effect developed in moist NADH preparations and that it is generated through a bimolecular reaction between NADH and NAD+ which in essence is a NAD+ catalyzed double bond rearrangement of NADH effected by transfer of hydride anions from NADH to NAD+. One implication of these findings, that removal of NAD+ from NADH preparations should convey increased stability to the reduced cofactor, has been verified. The structure of the lactate dehydrogenase inhibitors described in the literature is discussed. © 1979 Carlsberg Laboratory.

引用

页码：65 / 75

页数：11

共 19 条

[1]

BERRY AJ, 1973, CLIN CHEM, V19, P1255

[2]

BIELLMANN JF, BIOCHEMISTRY

[3] NATURAL ABUNDANCE C-13 NUCLEAR MAGNETIC-RESONANCE SPECTRA OF NICOTINAMIDE ADENINE-DINUCLEOTIDE AND RELATED NUCLEOTIDES [J].