TIME-RESOLVED FLUORESCENCE MICROSCOPY USING MULTICHANNEL PHOTON-COUNTING

被引：35

作者：

WANG, XF ^{[1
]}

KITAJIMA, S ^{[1
]}

UCHIDA, T ^{[1
]}

COLEMAN, DM ^{[1
]}

MINAMI, S ^{[1
]}

机构：

[1] OSAKA UNIV,FAC ENGN,DEPT APPL PHYS,SUITA,OSAKA 565,JAPAN

来源：

APPLIED SPECTROSCOPY | 1990年 / 44卷 / 01期

关键词：

D O I：

10.1366/0003702904085868

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A new measurement system is reported which combines fluorescence microscopy with multichannel photon-counting detection. The prototype instrument can be operated in normal fluorescence and in fluorescence lifetime modes. Multichannel photon detection was used in order to enhance the rate of data collection. The feasibility of rapid two-dimensional mapping of samples was indicated. The technique may prove especially valuable in situations where multicomponent samples display similar spectral features but differing lifetime characteristics. By increases in the measurement dimensionality, enhanced sample evaluation is possible. Applications involving model and practical biological systems are discussed.

引用

页码：25 / 30

页数：6

共 8 条

[1]

Berlman I. B., 1973, HDB FLUORESCENCE SPE

[2]

LAWTON M, 1976, J PHYS E SCI INSTRUM, V9, P686, DOI 10.1088/0022-3735/9/8/024

[3] STRUCTURE AND EXPRESSION OF GENES-CODING FOR XYLAN-DEGRADING ENZYMES OF BACILLUS-PUMILUS [J].

MORIYAMA, H ;

FUKUSAKI, E ;

CRESPO, JC ;

SHINMYO, A ;

OKADA, H .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 166 (03) :539-545

[4]

OConnor D. V. O., 1984, TIME CORRELATED SING

[5] AN INSTRUMENT FOR SIMULTANEOUS ACQUISITION OF FLUORESCENCE-SPECTRA AND FLUORESCENCE LIFETIMES FROM SINGLE CELLS [J].

RAMPONI, R ;

RODGERS, MAJ .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1987, 45 (01) :161-165

[6]

THAER AA, 1973, FLUORESCENCE TECHNIQ

[7] QUANTITATIVE MICROFLUOROMETRY OF ISOLATED LIVING CELLS WITH PULSED EXCITATION - DEVELOPMENT OF AN EFFECTIVE AND RELATIVELY INEXPENSIVE INSTRUMENT [J].

VIGO, J ;

SALMON, JM ;

VIALLET, P .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1987, 58 (08) :1433-1438

[8]

PRINCETON APPLIED RE

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