Fumonisin B1 (FB1) was isolated from samples of forage grass originating in paddocks associated with an idiopathic disease of Canadian wapiti and wapiti-red deer hybrids characterized by "ill thrift" and liver dysfunction. Four of 40 samples contained 1, 3, 6, and 9 ppm (micrograms per gram) of FB1 and 4, 0.5, 2, and 0.5 ppm, respectively, of the methyl ester of FB1. Analyses were done by ion spray mass spectrometry and confirmed by both fast atom bombardment (solids probe) and mass spectral analysis by electron impact ionization of the trifluoroacetate derivative of the base hydrolyzed product (pentolamine) of FB1. This article contains the first report of the presence of fumonisin B1 in grass.