RIBOSOMAL LOCALIZATION OF THE MESSENGER-RNA IN THE 30S INITIATION COMPLEX AS REVEALED BY UV CROSS-LINKING

被引：10

作者：

BRANDT, R

GUALERZI, CO

机构：

[1] UNIV CAMERINO,DEPT BIOL,GENET LAB,I-62032 CAMERINO,ITALY

[2] MAX PLANCK INST MOLEC GENET,W-1000 BERLIN,GERMANY

来源：

FEBS LETTERS | 1992年 / 311卷 / 03期

关键词：

TRANSLATION INITIATION; INITIATION COMPLEX; RIBOSOME; MESSENGER RNA; UV-CROSS-LINKING;

D O I：

10.1016/0014-5793(92)81101-Q

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Translation initiation complexes consisting of 30S ribosomal subunits, P-32-labelled mRNA (002 mRNA), fMet-tRNA and the three initiation factors were subjected to UV-crosslinking to determine the protein and rRNA neighbors of the bound mRNA by immunochemical methods and by nucleic acid hybridization techniques. The mRNA was found to be crosslinked to a specific region of the 16S rRNA spanning from nucleotide 418 to 615 and to ribosomal proteins S1 and S21 (the main targets), S3, S10, S12 and S14; a low level of crosslinking was also detected with S2, S7, S13, S18 and S19.

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页码：199 / 202

页数：4

相关论文

共 29 条

[1] SPATIAL-ORGANIZATION OF TEMPLATE POLYNUCLEOTIDES ON THE RIBOSOME DETERMINED BY FLUORESCENCE METHODS [J].

BAKIN, AV ;

BORISOVA, OF ;

SHATSKY, IN ;

BOGDANOV, AA .

JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (02) :441-453

[2]

BRANDT R, 1991, BIOCHIMIE, V173, P1543

[3] STRUCTURE-FUNCTION CORRELATIONS (AND DISCREPANCIES) IN THE 16S RIBOSOMAL-RNA FROM ESCHERICHIA-COLI [J].

BRIMACOMBE, R .

BIOCHIMIE, 1992, 74 (04) :319-326

[4] SELECTION OF THE MESSENGER-RNA TRANSLATION INITIATION REGION BY ESCHERICHIA-COLI RIBOSOMES [J].

CALOGERO, RA ;

PON, CL ;

CANONACO, MA ;

GUALERZI, CO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6427-6431

[5] ALTERNATIVE OCCUPANCY OF A DUAL RIBOSOMAL-BINDING SITE BY MESSENGER-RNA AFFECTED BY TRANSLATION INITIATION-FACTORS [J].

CANONACO, MA ;

GUALERZI, CO ;

PON, CL .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (03) :501-506

[6]

COOPERMAN BS, 1980, RIBOSOMES STRUCTURE, P531

[7] FLUORESCENCE STUDY OF THE TOPOLOGY OF MESSENGER-RNA BOUND TO THE 30S RIBOSOMAL-SUBUNIT OF ESCHERICHIA-COLI [J].

CZWORKOWSKI, J ;

ODOM, OW ;

HARDESTY, B .

BIOCHEMISTRY, 1991, 30 (19) :4821-4830

[8] THE LOCATION OF MESSENGER-RNA IN THE RIBOSOMAL 30S INITIATION COMPLEX - SITE-DIRECTED CROSS-LINKING OF MESSENGER-RNA ANALOGS CARRYING SEVERAL PHOTO-REACTIVE LABELS SIMULTANEOUSLY ON EITHER SIDE OF THE AUG START CODON [J].

DONTSOVA, O ;

KOPYLOV, A ;

BRIMACOMBE, R .

EMBO JOURNAL, 1991, 10 (09) :2613-2620

[9] LOCALIZATION OF 5'-END AND 3'-END OF THE RIBOSOME-BOUND SEGMENT OF TEMPLATE POLYNUCLEOTIDES BY IMMUNE ELECTRON-MICROSCOPY [J].

EVSTAFIEVA, AG ;

SHATSKY, IN ;

BOGDANOV, AA ;

SEMENKOV, YP ;

VASILIEV, VD .

EMBO JOURNAL, 1983, 2 (05) :799-804

[10] MAPPING OF CHLOROPLAST MUTATIONS CONFERRING RESISTANCE TO ANTIBIOTICS IN CHLAMYDOMONAS - EVIDENCE FOR A NOVEL SITE OF STREPTOMYCIN RESISTANCE IN THE SMALL SUBUNIT RIBOSOMAL-RNA [J].

GAUTHIER, A ;

TURMEL, M ;

LEMIEUX, C .

MOLECULAR & GENERAL GENETICS, 1988, 214 (02) :192-197