TARGETED DEVELOPMENT OF MICROSATELLITE MARKERS FROM INTER-ALU AMPLIFICATION OF YAC CLONES

被引：11

作者：

DESOUZA, AP

ALLAMAND, V

RICHARD, I

BRENGUIER, L

CHUMAKOV, I

COHEN, D

BECKMANN, JS

机构：

[1] FDN JEAN DAUSSET,CEPH,F-75010 PARIS,FRANCE

[2] UNIV ESTADUAL CAMPINAS,BR-6109 CAMPINAS,SP,BRAZIL

[3] GENETHON,F-91000 EVRY,FRANCE

来源：

GENOMICS | 1994年 / 19卷 / 02期

关键词：

D O I：

10.1006/geno.1994.1079

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Primary genetic maps based on highly informative markers are now available. The local density of these markers may, however, not be sufficient. There is thus a need for new means to generate polymorphic markers from targeted regions of the genome. This can be achieved by selectively cloning and sequencing (CA),positive human inter-ALu sequences from targeted YAC clones. This method was tested on 21 YACs and led to the development of seven new polymorphic microsatellite markers. (C) 1994 Academic Press, Inc.

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收藏

页码：391 / 393

页数：3

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