AN IMPROVED PROCEDURE FOR ASSAYING HATCHING ACTIVITY OF POTATO ROOT DIFFUSATES TOWARDS GLOBODERA-ROSTOCHIENSIS

被引：27

作者：

TWOMEY, U ^{[1
]}

RAFTERY, T ^{[1
]}

DEVINE, K ^{[1
]}

JONES, P ^{[1
]}

机构：

[1] NATL UNIV IRELAND UNIV COLL CORK,NATL FOOD BIOTECHNOL CTR,CORK,IRELAND

来源：

NEMATOLOGICA | 1995年 / 41卷 / 02期

关键词：

HATCHING FACTOR PURIFICATION; POTATO CYST NEMATODES;

D O I：

10.1163/003925995X00224

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

A bioassay for cyst nematode hatching activity is described which is applicable to purification research on hatching factors. Using an easily-constructed incubation unit, based on a 96-well microtitre plate, cysts were suspended, via ''thimbles'' of wire mesh, in test samples. The use of size-graded cyst samples reduced inter-replicate variability with respect to total egg content and hatching rate. The optimised bioassay procedure used 340 mu l test volumes and replicate sizes of five cysts, and the cumulative number of juveniles hatching per cyst after 5 days was closely positively correlated with final hatch (after 31 days). This procedure significantly reduces the labour, time and space requirements of hatching bioassay.

引用

页码：258 / 268

页数：11

共 9 条

[1] FENWICK D. W., 1958, JOUR HELMINTHOL, V32, P125, DOI 10.1017/S0022149X00019532
[2] HATCHING OF GLOBODERA-PALLIDA EGGS AFTER BRIEF EXPOSURES TO POTATO ROOT DIFFUSATE
FORREST, JMS
PERRY, RN
[J]. NEMATOLOGICA, 1980, 26 (01): : 130 - 132
[3] JONES F. G. W., 1962, NEMATOLOGICA, V8, P39
[4] ISOLATION OF A NATURAL HATCHING STIMULUS, GLYCINOECLEPIN-A, FOR THE SOYBEAN CYST NEMATODE
MASAMUNE, T
ANETAI, M
TAKASUGI, M
KATSUI, N
[J]. NATURE, 1982, 297 (5866) : 495 - 496
[5] PERRY R N, 1986, Revue de Nematologie, V9, P280
[6] SHEPHERD AM, 1959, NEMATOLOGICA, V2, P161
[7] STEELE AE, 1976, J NEMATOL, V8, P23
[8] COLLECTION AND IDENTIFICATION OF ALLELOPATHIC COMPOUNDS FROM THE UNDISTURBED ROOT-SYSTEM OF BIGALTA LIMPOGRASS (HEMARTHRIA-ALTISSIMA)
TANG, CS
YOUNG, CC
[J]. PLANT PHYSIOLOGY, 1982, 69 (01) : 155 - 160
[9] VIGLIERCHIO D, 1961, PHYTOPATHOLOGY, V51, P330

← 1 →