SEPARATION AND CHARACTERIZATION OF A GLYCOGENOLYTIC ENZYME FROM A GLUCOSE OXIDASE PREPARATION OBTAINED FROM ASPERGILLUS NIGER

被引：10

作者：

LUNDQUIST, I

LINDSTRAND, K

RERUP, C

机构：

[1] Department of Pharmacology and Department of Biochemistry, The Royal University, Lund

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1969年 / 23卷 / 01期

关键词：

Amyloglucosidase; Contaminant in glucose oxidase preparation; Enzymes; Glucose oxidase; Maltose; Separation glycogen;

D O I：

10.3109/00365516909078091

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

A glycogen cleaving factor was separated from commercial glucose oxidase by gel filtration on a combined column of Sephadex G 150 + G 25, and proved to be an enzyme. The enzyme was studied with purified glycogen and maltose as substrates and has been classified as an amyloglucosidase. Primary product of the reaction was found to consist exclusively of βglucose. pH optimum was 4.6, the temperature optimum 50° Several amines such as Tris, cetylpyridinium, and protamine inhibited the reaction at pH 7.0. Above separation procedure may be used for achievement of specificity for glucose determination by glucose oxidase method. © 1969 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：89 / +

页数：1

共 15 条

[1] DETERMINATION OF MALTASE AND ISOMALTASE ACTIVITIES WITH A GLUCOSE-OXIDASE REAGENT [J].