BASE-PAIRED INTERACTION, INVITRO, BETWEEN HEN GLOBIN 9S-MESSENGER RNA AND EUKARYOTIC RIBOSOMAL-RNAS

被引：15

作者：

AZAD, AA

DEACON, NJ

机构：

[1] Molecular Biology Unit, Research School of Biological Sciences, The Australian National University, A.C.T.

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 86卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)91751-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hen globin 9S mRNA complexes efficiently with mouse sarcoma 18S rRNA and to a lesser extent with 28S rRNA but not with tRNA. The mRNA-18S rRNA complex is dissociated under conditions that lead to disruption of H-bonds and exhibits a biphasic thermal denaturation curve with Tm(melting temperature) at .apprx. 39 and 58.degree. C in 0.15 M NaCl-0.03 M Tris-HCl, pH 7.5. Hen globin mRNA interacts with 18S rRNA from various other eukaryotes [hen reticulocytes, rabbits reticulocytes rat liver, barley and yeast]. The melting profile and TM of the complex formed with hen 18S rRNA is very similar to that of the complex formed with mouse sarcoma 18S rRNA.

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页码：568 / 576

页数：9

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