COMBINED AUTO-RADIOGRAPHY AND HISTOCHEMISTRY - SIMULTANEOUS DETECTION OF 6-H-3 THYMIDINE AND ACID-PHOSPHATASE ACTIVITY IN CRYOSTAT SECTIONS

被引：6

作者：

LEWIS, GHJ

BOWEN, ID

BELLAMY, D

机构：

[1] Department of Zoology, University College, Cardiff, CF1 1XL

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1979年 / 117卷 / NOV期

关键词：

D O I：

10.1111/j.1365-2818.1979.tb01181.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A method is described for the simultaneous demonstration of 6‐3H thymidine incorporation and acid phosphatase activity in cryostat sections of mouse thymus. The method enables a comparison of mitosis and acid hydrolase activity to be made in the same tissue section. In 8‐week‐old mice acid phosphatase positive cells represent 1·23 ±0·06% of the total population and 8·4 ± 0·27% of the cells incorporate tritiated thymidine. Acid phosphatase activity can be used to estimate cell autolysis and death. The implications of the method in relation to tissue dynamics is discussed. 1979 Blackwell Science Ltd

引用

页码：255 / 259

页数：5

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