ISOFORM PATTERNS OF APOLIPOPROTEIN-E IN DIABETES-MELLITUS

被引：17

作者：

BLACK, SC ^{[1
]}

HEWETT, S ^{[1
]}

KOTUBI, Y ^{[1
]}

BRUNT, RV ^{[1
]}

RECKLESS, JPD ^{[1
]}

机构：

[1] ROYAL UNITED HOSP,BATH INST CLIN SCI,DIABET & LIPID RES LAB,COMBE PK,BATH BA1 3NG,ENGLAND

来源：

DIABETIC MEDICINE | 1990年 / 7卷 / 06期

关键词：

Apolipoprotein E isoforms; Apolipoproteins; Diabetes mellitus; Glycosylation; Hyperlipidaemia; Very low density lipoproteins;

D O I：

10.1111/j.1464-5491.1990.tb01437.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Apolipoproteins in delipidated VLDL preparations from normal, diabetic, and non‐diabetic hyperlipidaemic subjects were analysed by SDS‐polyacrylamide gel electrophoresis, and by isoelectric focusing. On electrophoresis, diabetic VLDL contained more apolipoprotein E (17.3 ± 7.3 (± SD)%, n = 54) than did VLDL from hyperlipidaemic (13.4 ± 4.2%, n = 52; p < 0.005) or normal (12.4 ± 2.6%, n = 29; p < 0.001) subjects. Apolipoprotein E excess was also seen when subgroups were characterized by apolipoprotein E phenotype. In diabetic patients of E3/E3 phenotype, apolipoprotein E was 16.4 ± 6.0% (n = 25), compared with 12.9 ± 2.5% in control subjects (n = 14; p = 0.008). Acidic isoforms were more common in 44 diabetic patients with E3/E3 phenotype; E3, E2, E1, and E1′ as percentage of total E apolipoprotein were 58.3 ± 7.6, 24.5 ± 4.4, 13.7 ± 4.5, and 3.8 ± 4.3% respectively, compared with 63.5 ± 10.4 (p = 0.034), 19.1 ± 5.3 (p < 0.001), 13.7 ± 6.5 (NS), and 3.7 ± 2.1% (NS) in 21 normal subjects. In 31 diabetic patients, of apolipoprotein E3/E3 phenotype, E3, E2, E1 and E1′ were 60.2 ± 7.3, 23.4 ± 9.4, 11.1 ± 4.1, and 5.4 ± 3.7%, respectively, compared with 68.0 ± 7.1 (p < 0.001), 21.9 ± 6.4 (NS), 6.3 ± 3.9 (p < 0.001), and 3.7 ± 2.5 (p < 0.05)% in 32 hyperlipidaemic patients. Diabetic patients of E3/E2 phenotype showed less apolipoprotein E3 than normal or hyperlipidaemic subjects, with a similar trend for apolipoprotein E4 in those of E4/E3 phenotype. Increases in minor apolipoprotein E isoforms imply more post‐translational protein modification in people with diabetes. 1990 Diabetes UK

引用

页码：532 / 539

页数：8

共 43 条

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