COMPARISON OF POLYCLONAL ANTISERA AND ANTI-43KDA ANTIGEN MONOCLONAL-ANTIBODIES FOR THE CULTURE-AMPLIFIED ANTIGENIC DETECTION OF MYCOPLASMA-PNEUMONIAE BY IMMUNOBLOTTING

被引：3

作者：

CIMOLAI, N ^{[1
]}

MAH, D ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA,DEPT PATHOL,DIV MED MICROBIOL,VANCOUVER V6T 1W5,BC,CANADA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1991年 / 13卷 / 02期

关键词：

CULTURE AMPLIFICATION; DIAGNOSIS; INFECTION; MYCOPLASMA-PNEUMONIAE;

D O I：

10.1016/0167-7012(91)90012-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mycoplasma pneumoniae is a common etiologic agent of community-acquired respiratory disease whose rapid laboratory diagnosis is complicated. We developed a culture-amplified immunological antigen detection assay which employs a monoclonal antibody for the recognition of a polypeptide antigen of 43kDa in an immunoblotting technique. Polyclonal antisera to the same antigen were obtained from rabbit and hamster immunization with affinity chromatography-purified antigen. The latter sera, sera from natural human infection and experimental hamster infection, and a pool of anti-43kDa monoclonal antibodies were compared to the single anti-43kDa monoclonal antibody, OC2F5. Among these, only polyclonal hamster anti-43kDa sera were able to enhance antigen detection quantitatively. This enhancement however did not result in a significant benefit to the culture-amplified system.

引用

页码：123 / 133

页数：11

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