DIFFERENTIAL EXPRESSION OF 2 FORMS OF GABA-A RECEPTOR GAMMA-2-SUBUNIT IN MICE

被引:38
作者
WANG, JB [1 ]
BURT, DR [1 ]
机构
[1] UNIV MARYLAND,SCH MED,DEPT PHARMACOL & EXPTL THERAPEUT,655 W BALTIMORE ST,BALTIMORE,MD 21201
关键词
GABA-A RECEPTOR; RNASE PROTECTION ASSAY; GENE EXPRESSION; RECEPTOR REGULATION; MOUSE BRAIN; PROTEIN KINASE-C; PHOSPHORYLATION;
D O I
10.1016/0361-9230(91)90054-N
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The gamma-aminobutyric acid type A (GABA(A)) receptor gamma-2-subunit is important for benzodiazepine action. In previous studies, two forms of gamma-2-subunit have been found which are generated by RNA alternative splicing (gamma-2L and gamma-2S) and differ by the presence or absence of an 8-amino acid insertion in the major intracellular loop between proposed transmembrane domains M3 and M4. We have used the RNase protection assay to study regulation of expression of the two forms of gamma-2-subunit in mouse brain, as judged from mRNA levels. We find that gamma-2L and gamma-2S are differentially expressed in brain regions and that gamma-2S is expressed at a fairly constant level during brain development while gamma-2L increases dramatically with maturation. Their differentially regulated expression suggests further that gamma-2L and gamma-2S form receptors with important functional differences.
引用
收藏
页码:731 / 735
页数:5
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