ANTICODON-INDEPENDENT AMINOACYLATION OF AN RNA MINIHELIX WITH VALINE

被引：78

作者：

FRUGIER, M ^{[1
]}

FLORENTZ, C ^{[1
]}

GIEGE, R ^{[1
]}

机构：

[1] CNRS, INST BIOL MOLEC & CELLULAIRE, BIOCHIM LAB, 15 RUE RENE DESCARTES, F-67084 STRASBOURG, FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 09期

关键词：

TRANSFER RNA IDENTITY; VALYLATION; VALYL-TRANSFER RNA SYNTHETASE;

D O I：

10.1073/pnas.89.9.3990

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Minihelices mimicking the amino acid acceptor and anticodon branches of yeast tRNA(Val) have been synthesized by in vitro transcription of synthetic templates. It is shown that a minihelix corresponding to. the amino acid acceptor branch and containing solely a valine-specific identity nucleotide can be aminoacylated by yeast valyl-tRNA synthetase. Its charging ability is lost after mutating this nucleotide. This ability is stimulated somewhat by the addition of a second hairpin helix that mimicks the anticodon arm, which suggests that information originating from the anticodon stem-loop can be transmitted to the active site of the enzyme by the core of the protein.

引用

页码：3990 / 3994

页数：5

共 36 条

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