COMPOSITIONAL COMPARTMENTALIZATION OF THE NUCLEAR GENOMES OF TRYPANOSOMA-BRUCEI AND TRYPANOSOMA-EQUIPERDUM

被引：10

作者：

ISACCHI, A ^{[1
]}

BERNARDI, G ^{[1
]}

机构：

[1] INST JACQUES MONOD,GENET MOLEC LAB,F-75005 PARIS,FRANCE

来源：

FEBS LETTERS | 1993年 / 335卷 / 02期

关键词：

ISOCHORE; TRYPANOSOME; GENOME COMPARTMENTALIZATION;

D O I：

10.1016/0014-5793(93)80725-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

High molecular weight DNA preparations from Trypanosoma brucei and Trypanosoma equiperdum were fractionated by preparative centrifugation in a Cs2SO4 density gradient in the presence of BAMD, bis(acetatomercurimethyl)dioxane, a sequence-specific DNA ligand. Analytical centrifugation in CsCl of the DNA fractions so obtained showed that both DNAs had a bimodal distribution with two major peaks banding at 1.702-1.703 and 1.708 g/cm(3) and representing 1/3 and 2/3 of total DNA, respectively. Several minor components were also detected. These results indicate that a compositional compartmentalization is not only found in the genome of vertebrates and plants, as already described, but also in those of protozoa such as Trypanosomes.

引用

页码：181 / 183

页数：3

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