THE MARINE LICHEN LICHINA-CONFINIS (OF-MULL) C-AG - ULTRASTRUCTURE AND LOCALIZATION OF NITROGENASE, GLUTAMINE-SYNTHETASE, PHYCOERYTHRIN AND RIBULOSE 1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE IN THE CYANOBIONT

被引:14
作者
JANSON, S [1 ]
RAI, AN [1 ]
BERGMAN, B [1 ]
机构
[1] NE HILL UNIV,DEPT BIOCHEM,SHILLONG 793014,INDIA
关键词
LICHINA-CONFINIS; CALOTHRIX; SYMBIOSIS; CYANOBACTERIUM; GLUTAMINE SYNTHETASE;
D O I
10.1111/j.1469-8137.1993.tb03805.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Thallus ultrastructure and the localization of proteins involved in photosynthesis, N2 fixation and ammonia assimilation was studied in the Lichina confinis (O. F. Mull) C. Ag.-Calothrix symbiosis. The lichen thallus showed profuse branching. Within this, the cyanobiont was located in the cortical zone. Haustoria were frequently observed in contact with vegetative cells of the cyanobiont but not with heterocysts. An extensive mucilage layer containing numerous vesicles occurred around cyanobiont cells. Immunolabelling showed nitrogenase to be exclusively located in heterocysts of the cyanobiont and the free-living Calothrix sp. Glutamine synthetase (GS) levels in the cyanobiont were found to be drastically decreased compared to the levels in free-living (cultured) Calothrix sp. Such a decrease was more prominent in mature parts of the thallus than in the apical region. Rubisco was mostly located in carboxysomes of the vegetative cells of the cyanobiont. There were relatively low levels of Rubisco in the cytoplasm of vegetative cells, while heterocysts showed negligible levels. In free-living (cultured) Calothrix sp., phycoerythrin (PE) was found to be located along the thylakoid membranes both in vegetative cells and heterocysts. Cyanobiont cells showed a similar pattern but the levels were relatively lower.
引用
收藏
页码:149 / 160
页数:12
相关论文
共 43 条
[1]  
AHMADJIAN V, 1962, PHYSIOLOGY BIOCHEMIS, P817
[2]  
Ahmadjian V., 1982, PROGR PHYCOLOGICAL R, P179
[3]  
[Anonymous], 1982, BIOL CYANOBACTERIA
[4]   ULTRASTRUCTURE OF STIGONEMA IN THE CEPHALODIA OF STEREOCAULON-PASCHALE [J].
BERGMAN, B ;
HUSSDANELL, K .
LICHENOLOGIST, 1983, 15 :181-190
[5]   THE NOSTOC-NEPHROMA SYMBIOSIS - LOCALIZATION, DISTRIBUTION PATTERN AND LEVELS OF KEY PROTEINS INVOLVED IN NITROGEN AND CARBON METABOLISM OF THE CYANOBIONT [J].
BERGMAN, B ;
RAI, A .
PHYSIOLOGIA PLANTARUM, 1989, 77 (02) :216-224
[6]   IMMUNO-GOLD LOCALIZATION OF GLUTAMINE-SYNTHETASE IN A NITROGEN-FIXING CYANOBACTERIUM (ANABAENA-CYLINDRICA) [J].
BERGMAN, B ;
LINDBLAD, P ;
PETTERSSON, A ;
RENSTROM, E ;
TIBERG, E .
PLANTA, 1985, 166 (03) :329-334
[7]   NITROGENASE IN FREE-LIVING AND SYMBIOTIC CYANOBACTERIA - IMMUNOELECTRON MICROSCOPIC LOCALIZATION [J].
BERGMAN, B ;
LINDBLAD, P ;
RAI, AN .
FEMS MICROBIOLOGY LETTERS, 1986, 35 (01) :75-78
[8]  
Braun-Howland E. B., 2018, CRC HDB SYMBIOTIC CY
[9]   DINITROGENASE REDUCTASE (FE-PROTEIN) OF NITROGENASE IN THE CYANOBACTERIAL SYMBIONTS OF 3 AZOLLA SPECIES - LOCALIZATION AND SEQUENCE OF APPEARANCE DURING HETEROCYST DIFFERENTIATION [J].
BRAUNHOWLAND, EB ;
LINDBLAD, P ;
NIERZWICKIBAUER, SA ;
BERGMAN, B .
PLANTA, 1988, 176 (03) :319-322
[10]   STUDIES ON THE ULTRASTRUCTURE OF SOME CYANOLICHEN HAUSTORIA [J].
BUDEL, B ;
RHIEL, E .
PROTOPLASMA, 1987, 139 (2-3) :145-152