BIODEGRADABLE POLYMER SCAFFOLDS FOR TISSUE ENGINEERING

被引：899

作者：

FREED, LE ^{[1
]}

VUNJAKNOVAKOVIC, G ^{[1
]}

BIRON, RJ ^{[1
]}

EAGLES, DB ^{[1
]}

LESNOY, DC ^{[1
]}

BARLOW, SK ^{[1
]}

LANGER, R ^{[1
]}

机构：

[1] ALBANY INT RES CO,MANSFIELD,MA 02048

来源：

BIO-TECHNOLOGY | 1994年 / 12卷 / 07期

关键词：

D O I：

10.1038/nbt0794-689

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Synthetic polymer scaffolds designed for cell transplantation were reproducibly made on a large scale and studied with respect to biocompatibility, structure and biodegradation rate. Polyglycolic acid (PGA) was extruded and oriented to form 13 mu m diameter fibers with desired tenacity. Textile processing techniques were used to produce fibrous scaffolds with a porosity of 97% and sufficient structural integrity to maintain their dimensions when seeded with isolated cartilage cells (chondrocytes) and cultured in vitro at 37 degrees C for 8 weeks. Cartilaginous tissue consisting of glycosaminoglycan and collagen was regenerated in the shape of the original PGA scaffold. The resulting cell-polymer constructs were the largest grown in vitro to date (1 cm diameter x 0.35 cm thick). Construct mass was accurately predicted by accounting for accumulation of tissue components and scaffold degradation. The scaffold induced chondrocyte differentiation with respect to morphology and phenotype and represents a model cell culture substrate that may be useful for a variety of tissue engineering applications.

引用

页码：689 / 693

页数：5

共 38 条

[1] POLYMERS FOR ABSORBABLE SURGICAL SUTURES .1. [J].