CULTURED RAT STRIATAL AND CORTICAL ASTROCYTES PROTECT MESENCEPHALIC DOPAMINERGIC-NEURONS AGAINST HYDROGEN-PEROXIDE TOXICITY INDEPENDENT OF THEIR EFFECT ON NEURONAL DEVELOPMENT

被引：85

作者：

LANGEVELD, CH

JONGENELEN, CAM

SCHEPENS, E

STOOF, JC

BAST, A

DRUKARCH, B

机构：

[1] VRIJE UNIV AMSTERDAM,NEUROSCI RES INST,GRAD SCH NEUROSCI AMSTERDAM,DEPT NEUROL,1081 BT AMSTERDAM,NETHERLANDS

[2] VRIJE UNIV AMSTERDAM,DEPT PHARMACOCHEM,DIV MOLEC PHARMACOL,LEIDEN AMSTERDAM CTR DRUG RES,1081 HV AMSTERDAM,NETHERLANDS

来源：

NEUROSCIENCE LETTERS | 1995年 / 192卷 / 01期

关键词：

DOPAMINERGIC NEURONS; ASTROCYTES; COCULTURE; DOPAMINE; OXIDATIVE STRESS; NEUROTOXICITY; NEUROPROTECTION;

D O I：

10.1016/0304-3940(95)11596-O

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Reactive oxygen species (ROS), including hydrogen peroxide, are supposed to be involved in the degeneration of dopaminergic neurons in Parkinson's disease. The potential role of astrocytes against neurotoxic effects of ROS was studied in cocultures of rat mesencephalic neurons and rat striatal or cortical astrocytes. Neuronal [H-3]dopamine uptake, a marker of dopaminergic neuron integrity, was enhanced by striatal astrocytes, but not by cortical astrocytes, compared to uptake in mesencephalic neurons cultured alone. Whereas hydrogen peroxide at concentrations up to 100 mu M reduced the [H-3]dopamine uptake in neuronal cultures, no reduction of the uptake was observed in cocultures, regardless of the origin of the supporting astrocytes. These results suggest that astrocyte mediated protection of neurons against hydrogen peroxide induced toxicity is not directly related to a region-specific neurotrophic effect.

引用

页码：13 / 16

页数：4

共 17 条

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