PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP) MOBILIZES INTRACELLULAR FREE CALCIUM IN CULTURED RAT TYPE-2, BUT NOT TYPE-1, ASTROCYTES

被引:26
作者
TATSUNO, I
ARIMURA, A
机构
[1] TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LABS,BELLE CHASSE,LA 70037
[2] CHIBA UNIV,SCH MED,DEPT INTERNAL MED 2,DIV ENDOCRINOL & METAB,CHUO KU,CHIBA 260,JAPAN
[3] TULANE UNIV,SCH MED,DEPT MED,NEW ORLEANS,LA 70112
[4] TULANE UNIV,SCH MED,DEPT ANAT & PHYSIOL,NEW ORLEANS,LA 70112
关键词
ASTROCYTE; CALCIUM MOBILIZATION; PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE; INTRACELLULAR COMMUNICATION; 2ND-MESSENGER;
D O I
10.1016/0006-8993(94)90790-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We investigated the effects of pituitary adenylate cyclase-activating polypeptide (PACAP38) on cytosolic free calcium ([Ca2+](i)) at a single cell level in both type-1 and type-2 cultured rat astrocytes using a calcium-sensitive fluorescent dye, fura-2. Type-1 astrocytes were relatively silent during the 20 min observation of baseline [Ca2+](i) and PACAP38 did not alter [Ca2+](i) in type-1 astrocytes at concentrations up to 1 mu M. In contrast, type-2 astrocytes could be divided into three subtypes (silent type, randomly-firing type and oscillatory type) based on the observation of baseline [Ca2+](i). Of 166 type-2 astrocytes tested, 145 (87.4%) were the silent type (stable basal [Ca2+](i) levels) and 13 (7.8%) were the randomly-firing type (random increases in [Ca2+](i)). PACAP38 could stimulate [Ca2+](i) in subpopulations of all three subtypes. In the silent type-2 astrocytes (4 experiments with 255 cells/experiment), 54.4 +/- 3.6% of the total number responded to PACAP38. The half maximal concentration (ED(50) of PACAP38 was 2.89 +/- 1.89 nM. Removing Ca2+ from the superfusion media did not alter the PACAP38-induced increase of [Ca2+](i). Neither 1-30 mu M of forskolin nor 1-10 mM of dibutyryl cyclic AMP increased [Ca2+](i) in the same type-2 astrocytes which responded to PACAP38. These findings suggest that PACAP increases [Ca2+](i) in type-2, not type-1, rat astrocytes by mobilizing Ca2+ from intracellular stores, and that this action is not linked to activation of the cyclic AMP second messenger system.
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页码:1 / 10
页数:10
相关论文
共 53 条
[1]   DIFFERENTIATION OF BIPOTENTIAL GLIAL PRECURSORS INTO OLIGODENDROCYTES IS PROMOTED BY INTERACTION WITH TYPE-1 ASTROCYTES IN CEREBELLAR CULTURES [J].
ALOISI, F ;
AGRESTI, C ;
DURSO, D ;
LEVI, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (16) :6167-6171
[2]  
ARIMURA A, 1992, REGUL PEPTIDES, V37, P287
[3]  
ARIMURA A, IN PRESS ANN NY ACAD
[4]   ION CHANNELS IN VERTEBRATE GLIA [J].
BARRES, BA ;
CHUN, LLY ;
COREY, DP .
ANNUAL REVIEW OF NEUROSCIENCE, 1990, 13 :441-474
[5]   COOPERATION BETWEEN 2 GROWTH-FACTORS PROMOTES EXTENDED SELF-RENEWAL AND INHIBITS DIFFERENTIATION OF OLIGODENDROCYTE TYPE-2 ASTROCYTE (O-2A) PROGENITOR CELLS [J].
BOGLER, O ;
WREN, D ;
BARNETT, SC ;
LAND, H ;
NOBLE, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (16) :6368-6372
[6]   EXCITATORY AMINO-ACIDS DIRECTLY DEPOLARIZE RAT-BRAIN ASTROCYTES IN PRIMARY CULTURE [J].
BOWMAN, CL ;
KIMELBERG, HK .
NATURE, 1984, 311 (5987) :656-659
[7]   NEURONAL CELL KILLING BY THE ENVELOPE PROTEIN OF HIV AND ITS PREVENTION BY VASOACTIVE INTESTINAL PEPTIDE [J].
BRENNEMAN, DE ;
WESTBROOK, GL ;
FITZGERALD, SP ;
ENNIST, DL ;
ELKINS, KL ;
RUFF, MR ;
PERT, CB .
NATURE, 1988, 335 (6191) :639-642
[8]   STIMULATORY ACTION OF PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP) ON THYROID-GLAND [J].
CHEN, W ;
INUI, T ;
HACHIYA, T ;
OCHI, Y ;
NAKAJIMA, Y ;
KAJITA, Y .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (02) :923-929
[9]   GLUTAMATE INDUCES CALCIUM WAVES IN CULTURED ASTROCYTES - LONG-RANGE GLIAL SIGNALING [J].
CORNELLBELL, AH ;
FINKBEINER, SM ;
COOPER, MS ;
SMITH, SJ .
SCIENCE, 1990, 247 (4941) :470-473
[10]   PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP) POTENTIATES THE GONADOTROPIN-RELEASING ACTIVITY OF LUTEINIZING-HORMONE-RELEASING HORMONE [J].
CULLER, MD ;
PASCHALL, CS .
ENDOCRINOLOGY, 1991, 129 (04) :2260-2262