LOCALIZATION OF THE CHOLINE-ACETYLTRANSFERASE (CHAT) GENE TO HUMAN CHROMOSOME-10

被引：11

作者：

COHENHAGUENAUER, O

BRICE, A

BERRARD, S

VANCONG, N

MALLET, J

FREZAL, J

机构：

[1] HOP NECKER ENFANTS MALAD,CLIN MAURICE LAMY,INSERM,U12,UNITE RECH GENET MED,F-75743 PARIS 15,FRANCE

[2] CNRS,DEPT GENET MOLEC,NEUROBIOL CELLULAIRE & MOLEC LAB,F-91190 GIF SUR YVETTE,FRANCE

来源：

GENOMICS | 1990年 / 6卷 / 02期

关键词：

D O I：

10.1016/0888-7543(90)90579-J

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cDNA clone encoding the complete sequence of porcine choline acetyltransferase (CHAT) isolated by S. Berrard et al. (1987, Proc. Natl. Acad. Sci. USA 84: 9280-9284) was hybridized to TaqI digests of a panel of 25 human-rodent somatic cell hybrids and to a complementary panel of 10 human-rodent hybrids in order to determine the chromosomal localization of human CHAT. To enhance the detection of the human signal, hybridization and washings were performed under low stringency conditions on membranes presaturated with sonicated DNA from parental rodent strains. All informative human fragments had the same distribution among the hybrids, mapping CHAT to a single human chromosome. CHAT was assigned to chromosome 10 because all other chromosomes were eliminated by exclusion based on the analysis of the signal segregation. This result indicates that mutation of the CHAT gene cannot be responsible for the primary defect in familial Alzheimer's disease. © 1990.

引用

页码：374 / 378

页数：5

共 13 条

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