OPTIMIZATION OF DATA-ACQUISTION RATES IN TIME-CORRELATED SINGLE-PHOTON FLUORIMETRY

被引：38

作者：

HAUGEN, GR ^{[1
]}

WALLIN, BW ^{[1
]}

LYTLE, FE ^{[1
]}

机构：

[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907

来源：

REVIEW OF SCIENTIFIC INSTRUMENTS | 1979年 / 50卷 / 01期

关键词：

D O I：

10.1063/1.1135671

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Four different operating configurations of a time-to-amplitude converter were examined for performance in the time-correlated single-photon fluorimetric experiment. Parameters considered were the intensity/count linearity, maximum data rate, and decay distortion. The inverted configuration with an interactive source was optimum. This arrangement combined total linearity with a 37-kHz conversion rate and did not distort the fluorescence decay. In addition, the pulse-height analyzer could severely limit the overall system data rate and could destroy the TAC intensity/count linearity.

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页码：64 / 72

页数：9

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