APPLICATION OF A BIOLUMINESCENCE ATP ASSAY IN BREWERY WASTEWATER-TREATMENT STUDIES

被引：21

作者：

HYSERT, DW ^{[1
]}

KNUDSEN, FB ^{[1
]}

MORRISON, NM ^{[1
]}

VANGHELUWE, G ^{[1
]}

LOM, T ^{[1
]}

机构：

[1] MOLSONS BREWERY LTD,BARRIE L4M 4T2,ONTARIO,CANADA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 07期

关键词：

D O I：

10.1002/bit.260210716

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The importance of having a rapid method for determining the viable biomass in activated‐sludge wastewater treatment plants (WWTP) for process control and development is well recognized. The firefly bioluminescence ATP assay has been proposed for this purpose. Such an assay using partially purified firefly luciferase and synthetic firefly luciferin for the bioluminescence reaction, a liquid scintillation counter in the out‐of‐coincidence mode as luminescence detector, and a sludge ATP extraction technique involving dimethyl sulfoxide at room temperature is described. Experiments with several pure bacteria cultures were done to demonstrate the feasibility of applying this assay to activated sludge to activated sludge WWTP investigation and control. The ATP content of samples taken from various points in a 350000 gal/day brewery activated‐sludge WWTP was monitored for 4.5 months. Good linear correlation between ATP and mixed‐liquor suspended solids, return sludge suspended solids, and effluent suspended solids were observed. Percentage viabilities of the various sludge samples were derived from the ATP results. Copyright © 1979 John Wiley & Sons, Inc.

引用

页码：1301 / 1314

页数：14

共 20 条

[1] ADENYLATE ENERGY CHARGE IN ESCHERICHIA-COLI DURING GROWTH AND STARVATION [J].

CHAPMAN, AG ;

FALL, L ;

ATKINSON, DE .

JOURNAL OF BACTERIOLOGY, 1971, 108 (03) :1072-+

[2]

CHAPPELLE EW, 1975, X70926 TM NASA

[3]

CHIU SY, 1973, J WATER POLLUT CON F, V45, P1746

[4]

GENETELLI EJ, 1971, WATER WATER POLLUTIO, V1, P415

[5]

Hysert D. W., 1976, Journal of the American Society of Brewing Chemists, V34, P145

[6]

Hysert D. W., 1977, Journal of the American Society of Brewing Chemists, V35, P160

[7]

JENKINS D, 1963, BIOCH IND MICROORGAN, P508

[8]

KAO IC, 1973, J WATER POLLUT CON F, V45, P926

[9]

KNUST EA, 1975, ANAL APPLICATIONS BI, P27

[10]

LESEELLEUR LA, 1971, TECH Q MASTER BREW A, V8, P52

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