COLD-SENSITIVE BETA-SUBUNIT MUTANT RNA-POLYMERASE FROM ESCHERICHIA-COLI WITH DEFECTS IN PROMOTER OPENING INVITRO

被引：12

作者：

LARIONOV, OA ^{[1
]}

GRAGEROV, AI ^{[1
]}

KALYAEVA, ES ^{[1
]}

NIKIFOROV, VG ^{[1
]}

机构：

[1] ACAD SCI USSR,INST MOLEC GENET,MOSCOW V-71,USSR

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 176卷 / 01期

关键词：

D O I：

10.1007/BF00334301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cold-sensitive mutation in the rpoB gene for the RNA polymerase β subunit increasing the temperature of promoter opening on T2 phage DNA was obtained in Escherichia coli. The mutation also affects the stages preceding promoter opening by increasing the dissociation rate of RNA polymerase-DNA closed complexes. The affinity of RNA polymerase to T2 and λ DNA is differentially changed by the mutation. The relative efficiency of transcription of these two templates is also changed. These results suggest a participation of the RNA polymerase β subunit in the interaction with promoters. © 1979 Springer-Verlag.

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页码：105 / 111

页数：7

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