T-CELL RECEPTOR ALPHA-CHAIN PLAYS A CRITICAL ROLE IN ANTIGEN-SPECIFIC SUPPRESSOR-CELL FUNCTION

被引:64
作者
KUCHROO, VK [1 ]
BYRNE, MC [1 ]
ATSUMI, Y [1 ]
GREENFIELD, E [1 ]
CONNOLLY, JB [1 ]
WHITTERS, MJ [1 ]
OHARA, RM [1 ]
COLLINS, M [1 ]
DORF, ME [1 ]
机构
[1] GENET INST,CAMBRIDGE,MA 02140
关键词
SUPPRESSOR FACTOR; T-CELL RECEPTOR MUTANTS; IMMUNOREGULATION; T-CELL HYBRIDOMAS;
D O I
10.1073/pnas.88.19.8700
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Antigen-specific suppressor T-cell hybridomas release soluble suppressor factors (TsF) in the supernatant that modulate both in vivo delayed-type hypersensitivity and in vitro plaque-forming cell responses in an antigen-specific manner. To study the relationship between the T-cell receptor (TcR) and TsF, we developed a series of TcR alpha- or TcR beta- expression variants from suppressor T-cell hybridomas that expressed the CD3-TcR alpha/beta complex. We demonstrate that loss of TcR alpha but not TcR beta mRNA was accompanied by the concomitant loss of suppressor bioactivity. Homologous transfection of TcR alpha cDNA into a TcR alpha-beta+ clone reconstituted both CD3-TcR expression and suppressor function. Furthermore, suppressor activity from TcR beta- variants was specifically absorbed by antigen and anti-TcR alpha antibodies, but not by anti-CD3 or anti-TcR beta affinity columns. These data directly establish a role for the TcR alpha chain in suppressor T-cell function and suggest that the TcR alpha chain is part of the antigen-specific TsF molecule.
引用
收藏
页码:8700 / 8704
页数:5
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