INHIBITION OF TRYPSIN BY THE BETA-AMYLOID PROTEIN-PRECURSOR - A COMPARATIVE-STUDY BETWEEN TRANSFECTED CELLS, HUMAN BRAIN AND CEREBROSPINAL-FLUID

被引：8

作者：

DELVAUX, A ^{[1
]}

VANDERELST, L ^{[1
]}

OCTAVE, JN ^{[1
]}

机构：

[1] CATHOLIC UNIV LOUVAIN,NEUROCHIM LAB,UCL 3031,CLOS CHAPELLE AUX CHAMPS 30,B-1200 BRUSSELS,BELGIUM

来源：

FEBS LETTERS | 1992年 / 297卷 / 1-2期

关键词：

BETA-AMYLOID PRECURSOR; CHARGE SEPARATION; INHIBITION OF SERINE PROTEASE;

D O I：

10.1016/0014-5793(92)80341-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Soluble beta-amyloid protein precursors (beta-APPs) were studied in human brain and cerebrospinal fluid (CSF) after partial purification by ion exchange chromatography. Proteins were analysed in immunoblotting experiments using a monoclonal antibody directed against the N-terminal segment of the beta-APP 770, and by reverse enzymography. In the human brain and CSF, a protein which comigrates with the beta-APP 770 expressed by transfected CHO cells was able to inhibit trypsin.

引用

页码：124 / 126

页数：3

共 23 条

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