ASSAY OF NICERGOLINE AND 3 METABOLITES IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE IONIZATION MASS-SPECTROMETRY

被引：19

作者：

BANNO, K

HORIMOTO, S

MABUCHI, M

机构：

[1] Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Yodogawaku, Osaka, 16-89

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80175-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the simultaneous determination of nicergoline and three of its metabolites in human plasma and urine has been developed using high-performance liquid chromatography-atmospheric pressure ionization mass spectrometry. Nicergoline and its metabolites were extracted from the plasma and urine samples with chloroform and separated on a reversed-phase ODS column. The eluents were led to the atmospheric pressure ionization interface and then analysed in the selected-ion monitoring mode. The detection limits of nicergoline and three of its metabolites were ca. 2 ng/ml in plasma and ca. 10 ng/ml in urine, at a signal-to-noise ratio of 4.

引用

页码：375 / 384

页数：10

共 19 条

[1] STUDIES ON METABOLISM OF ERGOLINE DERIVATIVES - METABOLISM OF NICERGOLINE IN MAN AND IN ANIMALS [J].