A PRODUCT OF PHOSPHATIDYLINOSITOL-3 KINASE IS ELEVATED IN DIVIDING HT29 COLONIC EPITHELIAL-CELLS

被引：13

作者：

DOBOS, GJ

WU, XR

TRAYNORKAPLAN, A

机构：

[1] UNIV CALIF SAN DIEGO,MED CTR,DEPT MED,225 DICKINSON ST,SAN DIEGO,CA 92103

[2] UNIV HOSP FREIBURG,DEPT MED,W-7800 FREIBURG,GERMANY

来源：

FEBS LETTERS | 1993年 / 324卷 / 02期

关键词：

PI-3; KINASE; PHOSPHATIDYLINOSITOL-3; PHOSPHATE; COLONIC EPITHELIA; CELL CYCLE;

D O I：

10.1016/0014-5793(93)81381-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphatidylinositol 3 kinase (PI-3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in Phosphoinositide metabolism during the cell cycle of HT-29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separated into mitotic and quiescent populations and their phospholipid composition was analyzed. Radiolabelled phospholipids from cells labelled with (PO4)-P-32 or [H-3]myoinositol were analyzed by TLC and/or deacylated and analyzed by HPLC. In all cases, levels of phosphatidylinositol 3 phosphate from mitotic phase cells were double that in resting populations. Therefore, levels of a product of PI-3 kinase are elevated and may play a role in cell division.

引用

页码：143 / 146

页数：4

共 18 条

[1] AUGER KR, 1989, J BIOL CHEM, V264, P20181
[2] ONCOGENES AND SIGNAL TRANSDUCTION
CANTLEY, LC
AUGER, KR
CARPENTER, C
DUCKWORTH, B
GRAZIANI, A
KAPELLER, R
SOLTOFF, S
[J]. CELL, 1991, 64 (02) : 281 - 302
[3] PP60C-SRC ACTIVATION IN HUMAN-COLON CARCINOMA
CARTWRIGHT, CA
KAMPS, MP
MEISLER, AI
PIPAS, JM
ECKHART, W
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1989, 83 (06) : 2025 - 2033
[4] ASSOCIATION OF TYPE-I PHOSPHATIDYLINOSITOL KINASE-ACTIVITY WITH MUTATIONALLY ACTIVATED FORMS OF HUMAN PP60C-SRC
CHAN, TO
TANAKA, A
BJORGE, JD
FUJITA, DJ
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (06) : 3280 - 3283
[5] THE USE OF SUBMICROSCOPIC GOLD PARTICLES COMBINED WITH VIDEO CONTRAST ENHANCEMENT AS A SIMPLE MOLECULAR PROBE FOR THE LIVING CELL
DEBRABANDER, M
NUYDENS, R
GEUENS, G
MOEREMANS, M
DEMEY, J
[J]. CELL MOTILITY AND THE CYTOSKELETON, 1986, 6 (02): : 105 - 113
[6] ENDEMANN G, 1990, J BIOL CHEM, V265, P396
[7] THE ACTIN-BINDING PROTEIN PROFILIN BINDS TO PIP2 AND INHIBITS ITS HYDROLYSIS BY PHOSPHOLIPASE-C
GOLDSCHMIDTCLERMONT, PJ
MACHESKY, LM
BALDASSARE, JJ
POLLARD, TD
[J]. SCIENCE, 1990, 247 (4950) : 1575 - 1578
[8] MODULATION OF GELSOLIN FUNCTION BY PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE
JANMEY, PA
STOSSEL, TP
[J]. NATURE, 1987, 325 (6102) : 362 - 364
[9] SPECIFIC INTERACTION BETWEEN PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE AND PROFILACTIN
LASSING, I
LINDBERG, U
[J]. NATURE, 1985, 314 (6010) : 472 - 474
[10] EGF INDUCES TYROSINE PHOSPHORYLATION OF PHOSPHOLIPASE C-II - A POTENTIAL MECHANISM FOR EGF RECEPTOR SIGNALING
MARGOLIS, B
RHEE, SG
FELDER, S
MERVIC, M
LYALL, R
LEVITZKI, A
ULLRICH, A
ZILBERSTEIN, A
SCHLESSINGER, J
[J]. CELL, 1989, 57 (07) : 1101 - 1107

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