DISTINGUISHING BETWEEN THE EXTRACELLULAR DNASES OF VIBRIO-CHOLERAE AND DEVELOPMENT OF A TRANSFORMATION SYSTEM

被引：51

作者：

FOCARETA, T ^{[1
]}

MANNING, PA ^{[1
]}

机构：

[1] UNIV ADELAIDE, DEPT MICROBIOL & IMMUNOL, GPO BOX 498, ADELAIDE, SA 5001, AUSTRALIA

来源：

MOLECULAR MICROBIOLOGY | 1991年 / 5卷 / 10期

关键词：

D O I：

10.1111/j.1365-2958.1991.tb02101.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Vibrio cholerae is known to secrete DNase(s) into the extracellular environment. These proteins have been thought to be responsible for the difficulties in transforming this organism. In this work we demonstrate that the dns and xds genes differ and that their products are solely responsible for the extracellular DNase activity. By site-directed mutagenesis, strains have been constructed which are mutant in one or both genes. These strains have been assessed for their ability to be transformed with plasmid DNA and for their virulence in the infant mouse cholera model. DNase-deficient mutants can be readily transformed and the product of dns appears to be the more significant barrier. No effect on virulence was observed with the mutants.

引用

页码：2547 / 2555

页数：9

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