EVALUATION OF PHAGOCYTIC FUNCTION OF CANINE PERIPHERAL POLYMORPHONUCLEAR LEUKOCYTES BY WHOLE-BLOOD CHEMILUMINESCENCE

被引：19

作者：

MAKIMURA, S ^{[1
]}

SAWAKI, M ^{[1
]}

机构：

[1] HITA RES LAB,INST CHEM INSPECT & TESTING,HITA 877,JAPAN

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1992年 / 54卷 / 01期

关键词：

CANINE WHOLE BLOOD CHEMILUMINESCENCE; PHAGOCYTOSIS; POLYMORPHONUCLEAR LEUKOCYTE; ZYMOSAN;

D O I：

10.1292/jvms.54.63

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Zymosan-induced and luminol-aided chemiluminescence (CL) of whole blood from beagle dogs was estimated for the function of polymorphonuclear leucocytes (PMNs). Whole blood (0.1 ml) was examined directly and results were obtained within 20 min. A phagocytic function of PMNs can be estimated from the peak CL counts and the number of PMNs in a specimen, and the opsonic activity can also be estimated by the peak time showing peak CL after the addition of non-opsonized zymosan. The optimal temperatures to keep diluted whole blood for the CL measurement was around 13-degrees-C. Thus, this method offers information concerning the functions of phagocytic cells in whole blood.

引用

页码：63 / 67

页数：5

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