ALMOST THE ENTIRE 5' NON-TRANSLATED REGION OF HEPATITIS-C VIRUS IS REQUIRED FOR CAP-INDEPENDENT TRANSLATION

被引：150

作者：

RIJNBRAND, R

BREDENBEEK, P

VANDERSTRAATEN, T

WHETTER, L

INCHAUSPE, G

LEMON, S

SPAAN, W

机构：

[1] LEIDEN UNIV,FAC MED,INST MED MICROBIOL,DEPT VIROL,2300 AH LEIDEN,NETHERLANDS

[2] UNIV N CAROLINA,DEPT MED,CHAPEL HILL,NC 27599

[3] NEW YORK BLOOD CTR,LINDSLEY F KIMBALL RES INST,NEW YORK,NY 10021

来源：

FEBS LETTERS | 1995年 / 365卷 / 2-3期

关键词：

HEPATITIS-C VIRUS; 5' UNTRANSLATED REGION; SECONDARY STRUCTURE; TRANSLATION; INTERNAL RIBOSOME ENTRY SITE; IRES; 2A;

D O I：

10.1016/0014-5793(95)00458-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To investigate which hairpin structures within the 5' untranslated region of hepatitis C virus (HCV) are necessary for cap-independent translation, mutants were constructed that lack one or more hairpin structures, Here we demonstrate, by constructing precisely defined hairpin deletion mutants, that with the exception of the most 5' located hairpin structure, which on deletion shows an increase on translation, each of the predicted hairpins is found to be essential for cap-independent translation, In addition, we demonstrate that HCV 5'UTR driven translation is stimulated by poliovirus 2A(pro) co-expression.

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页码：115 / 119

页数：5

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